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Progress in Aspergillus niger as cell factory for secretory proteins
Reviews and monographs | 更新时间：2026-07-24
|
Progress in
Aspergillus niger as cell factory for secretory proteins- CIESC Journal Vol. 70, Issue 10, Pages: 3690-3703(2019)
- 作者机构：
浙江大学教育部生物质化工重点实验室，化学工程与生物工程学院，浙江 杭州 310027
- 作者简介：
- 基金信息：
DOI：10.11949/0438-1157.20190591
CLC： TQ 028.8Received：29 May 2019，
Revised：2019-07-02，
Online First：24 July 2026，
Published：05 October 2019
- 稿件说明：
移动端阅览
姚善泾, 蔡礼年, 林东强. 黑曲霉作为分泌蛋白细胞工厂的研究进展[J]. 化工学报, 2019, 70(10): 3690-3703
YAO Shanjing, CAI Linian, LIN Dongqiang. Progress in Aspergillus niger as cell factory for secretory proteins[J]. CIESC Journal, 2019, 70(10): 3690-3703
姚善泾, 蔡礼年, 林东强. 黑曲霉作为分泌蛋白细胞工厂的研究进展[J]. 化工学报, 2019, 70(10): 3690-3703 DOI： 10.11949/0438-1157.20190591.
YAO Shanjing, CAI Linian, LIN Dongqiang. Progress in Aspergillus niger as cell factory for secretory proteins[J]. CIESC Journal, 2019, 70(10): 3690-3703 DOI： 10.11949/0438-1157.20190591.
Progress in Aspergillus niger as cell factory for secretory proteins
摘要
黑曲霉具有极强的分泌蛋白的能力，其分泌的木质纤维素降解酶、淀粉酶、蛋白酶、脂肪酶等广泛应用于食品、饲料和生物技术等相关工业中。本文围绕黑曲霉生产同源和异源分泌蛋白，阐述黑曲霉作为分泌蛋白细胞工厂的巨大潜力。首先，通过总结黑曲霉表达系统的优越性，确定了黑曲霉作为分泌蛋白细胞工厂的开发前景。随后，介绍了初步构建黑曲霉分泌蛋白细胞工厂的一般流程。接着，总结了近十年来黑曲霉作为同源分泌酶细胞工厂的研究进展，提出在黑曲霉中定向表达分泌酶是深入研究黑曲霉自身分泌酶性质、功能和结构的理想策略。最后，总结了近年来黑曲霉作为异源分泌蛋白细胞工厂的研究进展，并从异源蛋白表达中遇到的难点出发介绍了完善黑曲霉细胞工厂来提高异源蛋白产量的对策。
Abstract
Aspergillus niger has a strong ability to secrete proteins
and its secreted lignocellulose degrading enzymes
amylases
proteases
and lipases are widely used in related industries such as food
feed
and biotechnology. We describe its great potential as cell factory for secretory proteins based on the production of homologous and heterologous secretory proteins by
A. niger
. Firstly
the prospect of
A. niger
as cell factory for secretory proteins is ascertained based on the superiority of its expression system. Then the standard procedure for construction of
A. niger
cell factory for secretory proteins is introduced. Subsequently
the advances on
A. niger
as cell factory for homologous secretases in the past decade are summarized. It is proposed that the directed expression of inherent secretases in
A. niger
is an ideal strategy to study their properties
functions and structures. Finally
the advances on
A. niger
as cell factory for heterologous secretory proteins in recent years are summarized
and based on the difficulties encountered in the expression of heterologous proteins
the countermeasures to
improve the production of heterologous proteins by further improvement of
A. niger
cell factory are introduced.
关键词
Keywords
references
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