Optimization of extraction protocol of chlorophyll a from algae

Pengfei LI; Xin SUN; Li YANG; Feifei HE; Xu WANG

doi:10.11949/0438-1157.20190149

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Optimization of extraction protocol of chlorophyll a from algae

更新时间：2026-03-25

- Optimization of extraction protocol of chlorophyll a from algae
- CIESC Journal Vol. 70, Issue 9, Pages: 3421-3429(2019)
- 作者机构：
  
  西安建筑科技大学环境与市政工程学院,陕西,西安,710055
- 作者简介：
- 基金信息：
- DOI：10.11949/0438-1157.20190149
  CLC： TQ611.5
- Online First：05 September 2019，
  
  Published：05 September 2019
- 稿件说明：
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Pengfei LI, Xin SUN, Li YANG, et al. Optimization of extraction protocol of chlorophyll a from algae[J]. CIESC Journal, 2019, 70(9): 3421-3429.
DOI：

Pengfei LI, Xin SUN, Li YANG, et al. Optimization of extraction protocol of chlorophyll a from algae[J]. CIESC Journal, 2019, 70(9): 3421-3429. DOI： 10.11949/0438-1157.20190149.

摘要

叶绿素a是参与光合作用的基础物质，是衡量水体藻类生物量以及评价湖泊富营养化的重要指标之一。利用90%丙酮、无水乙醇、无水乙醇∶90%丙酮（1∶1）、无水乙醇∶90%丙酮（1∶2）、无水乙醇∶90%丙酮（2∶1）五种有机溶剂萃取已知浓度标准叶绿素a和叶绿素b，对传统萃取叶绿素a的计算公式加以修正，并且在超声辅助破碎藻细胞的前提下分析了该五种有机溶剂对普通小球藻和铜绿微囊藻中叶绿素a的萃取效果。确定了无水乙醇∶90%丙酮（2∶1）的萃取效果最好，而无水乙醇从效果、安全、环保等角度考虑为最优溶剂。通过多参数水质监测仪对不同时间段的浅水型湖库水体中的叶绿素a进行监测，验证了在超声条件下无水乙醇测定结果的准确性并利用正交实验确定出了无水乙醇萃取叶绿素a的最佳条件。对于叶绿素a测定的优化研究使得以后野外水体大量测量叶绿素a的过程变得更为简便、快捷、准确。

Abstract

Chlorophyll a is a basic substance involved in photosynthesis

and is one of the important indicators for measuring algae biomass in water and evaluating eutrophication of lakes. The traditional calculation formula for extracting chlorophyll a was modified when the standard chlorophyll a and chlorophyll b was extracted by these organic solvents including 90% acetone

anhydrous ethanol

anhydrous ethanol∶acetone (1∶1)

anhydrous ethanol∶acetone (1∶2) and anhydrous ethanol∶acetone (2∶1). The effects of these five organic solvents on Chlorella vulgaris and Microcystis aeruginosa were analyzed under the ultrasonic treatment. It was determined that anhydrous ethanol∶acetone (2∶1) was most effective for extraction and anhydrous ethanol was considered as the best solvent in terms of efficiency

safety and environmental protection. The chlorophyll a in shallow water reservoir was monitored by multi-parameter water quality monitor at different times. The accuracy of the determination results of anhydrous ethanol under ultrasonic treatment was verified. The optimum extraction conditions of chlorophyll a by anhydrous ethanol were determined by orthogonal experiment. The optimization of chlorophyll a measurement makes the process of measuring chlorophyll a for large quantities in water more simple

fast and accurate.

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