Preparation of macro-porous pellicular agarose-glass composite matrix and its application to protein adsorption

ZHOU Xin; SUN Yan; LIU Zheng

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Preparation of macro-porous pellicular agarose-glass composite matrix and its application to protein adsorption

更新时间：2026-03-26

- Preparation of macro-porous pellicular agarose-glass composite matrix and its application to protein adsorption
- CIESC Journal Vol. 57, Issue 2, (2006)
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- Online First：25 February 2006，
  
  Published：2006
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ZHOU Xin, SUN Yan, LIU Zheng. Preparation of macro-porous pellicular agarose-glass composite matrix and its application to protein adsorption[J]. CIESC Journal, 2006, 57(2).
DOI：

ZHOU Xin, SUN Yan, LIU Zheng. Preparation of macro-porous pellicular agarose-glass composite matrix and its application to protein adsorption[J]. CIESC Journal, 2006, 57(2). DOI：

摘要

采用油水乳化法，以环己烷和碳酸钙微粒复合致孔，以6%的琼脂糖凝胶包裹玻璃珠，制备出大孔薄层琼脂糖凝胶-玻璃珠复合介质，其密度为1.7 g8226;ml-1，平均粒径为127.8 μm，在其上接枝二乙胺基乙基制备成阴离子交换介质，进行了牛血清白蛋白的吸附实验.对比实验结果表明：此大孔薄层介质具有较好的吸附容量和良好的传质特性.

Abstract

A macro-porous pellicular agarose-glass composite matrix（MPAG）was prepared by coating 6% agarose gel onto glass beads by water-in-oil emulsification using a mixture of cyclohexane and fine calcium carbonate powder as porogenic diluents. The density of MPAG was 1.7 g8226;ml-1 and the average bead size was 127.8 μm. MPAG was derivatized with diethylaminoethyl(DEAE) and subjected to the adsorption of bovine serum albumin. Compared to macro-porous matrix or homogeneous matrix derivatized with DEAE

DEAD-MPAG gave a higher adsorption capacity at a faster mass transfer rate.

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