EXTRACTION OF PROTEIN WITH REVERSED MICELLES USING A PACKED SIEVEPLATE ROLL CONTACTOR

张伟; 刘会洲; 陈家镛

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EXTRACTION OF PROTEIN WITH REVERSED MICELLES USING A PACKED SIEVEPLATE ROLL CONTACTOR

更新时间：2025-12-16

- EXTRACTION OF PROTEIN WITH REVERSED MICELLES USING A PACKED SIEVEPLATE ROLL CONTACTOR
- CIESC Journal Issue S1, (2000)
- 作者机构：
  
  中国科学院化工冶金所分离科学与工程青年实验室、生化工程国家重点实验室!北京 100080,中国科学院化工冶金所分离科学与工程青年实验室、生化工程国家重点实验室!北京 100080,中国科学院化工冶金所分离科学与工程青年实验室、生化工程国家重点实验室,北京,100080
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q028
- Online First：30 December 2000，
  
  Published：30 December 2000
- 稿件说明：
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张伟, 刘会洲, 陈家镛. EXTRACTION OF PROTEIN WITH REVERSED MICELLES USING A PACKED SIEVEPLATE ROLL CONTACTOR[J]. CIESC Journal, 2000, (S1).
DOI：

张伟, 刘会洲, 陈家镛. EXTRACTION OF PROTEIN WITH REVERSED MICELLES USING A PACKED SIEVEPLATE ROLL CONTACTOR[J]. CIESC Journal, 2000, (S1). DOI：

摘要

设计出滚筒式填料筛板萃取器，并应用该萃取器反胶团法萃取蛋白质。萃取溶菌酶和牛血清白蛋白（ＢＳＡ）的实验结果表明，该萃取器萃取效果良好，分相迅速，可澄清分相的水相ＫＣＬ最低浓度为０．０２ｍｏｌ／Ｌ，可有效解决反胶团法萃取蛋白质过程分相困难的问题。报道使用石油醚为溶剂反胶团法萃取蛋白质。石油醚为混合烷烃，价格低廉，易于纯化，萃取效果良好，不会引起酶的失活。

Abstract

A packed sieveplate roll contactor was designed to extraction protein with reversed micelles. The experimental results indicated that the new contactor could solve the phase separation problem of reversed micelles. The lowest salt concentration operating the new contactor with reversed micelles was determined as 0.02mol/L KCI. Petroleum ether

which is much cheaper than pure straight chain hydrocarbons such as isooctane and he-cane and did not lead to denaturization of protein

could be used as solvent in protein eXtraction with reversed micelles.

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