ISOLATION OF A CHITOSANASE-PRODUCING PENICILLIUM STRAIN AND OPTIMIZATION OF ITS FERMENTATION CONDITIONS

Zhu Jiangfeng and Zheng  Lianying  (Department of Chemical and Biochemical  Engineering; Zhejiang  University Hangzhou 310027)

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ISOLATION OF A CHITOSANASE-PRODUCING PENICILLIUM STRAIN AND OPTIMIZATION OF ITS FERMENTATION CONDITIONS

更新时间：2025-12-16

- ISOLATION OF A CHITOSANASE-PRODUCING PENICILLIUM STRAIN AND OPTIMIZATION OF ITS FERMENTATION CONDITIONS
- CIESC Journal Issue S1, (2000)
- 作者机构：
  
  浙江大学化学工程与生物工程系!杭州310027,浙江大学化学工程与生物工程系,杭州,310027
- 作者简介：
- 基金信息：
- DOI：
  CLC： 939
- Online First：30 December 2000，
  
  Published：30 December 2000
- 稿件说明：
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Zhu Jiangfeng and Zheng Lianying (Department of Chemical and Biochemical Engineering, Zhejiang University Hangzhou 310027). ISOLATION OF A CHITOSANASE-PRODUCING PENICILLIUM STRAIN AND OPTIMIZATION OF ITS FERMENTATION CONDITIONS[J]. CIESC Journal, 2000, (S1).
DOI：

Zhu Jiangfeng and Zheng Lianying (Department of Chemical and Biochemical Engineering, Zhejiang University Hangzhou 310027). ISOLATION OF A CHITOSANASE-PRODUCING PENICILLIUM STRAIN AND OPTIMIZATION OF ITS FERMENTATION CONDITIONS[J]. CIESC Journal, 2000, (S1). DOI：

摘要

从自然界中采集土壤样品，经筛选获得了一株产高活性壳聚糖酶的菌种，经初步鉴定属于青霉属。对该菌种进行紫外光诱变提高了它的产酶能力。通过Ｐｌａｃｋｅｔｔ－Ｂｕｒｍａｎ设计初步优化了产酶条件，并研究了培养温度、摇床转速、培养基组成和装液量等培养条件对菌种产酶能力的影响，确定了该菌种的最佳产酶条件。在最佳培养条件下，壳聚精酶活最高可达２１６．６Ｕ／ｍｌ．

Abstract

A chitosanase-producing bacterium strain is isolated from soil. It was identified as Penicillium sp. Its chitosanase yield was improved by UV irradiation. The chitosanase fermentation condition for the mutant strain was first optimized with Plackett-Burman design and then the influence of the culture temperature

the rotating speed of the rime

the component of the culture medium and the medium volume were studied to optimize the fermentation conditions. A maximum yield of 216.6 U/ml was obtained.

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