Taking hepatcyote lines L-02 as materials

the fluorescence spectrum of the fluorescent probe 1

6 dipheny-1

3

5 hexatriene(DPH) used to label the fluidity of intact plasma membranes is studied. Furthermore

influence on the damage of intact plasma membranes induced by free radicals and the regulation of tea polyphenols is researched. The result shows that the volue fluorescence peaks transfers from 442um to 432um

but the fluorescence Ploarization of cells membranes is stable betWeen 0.277 and 0.280 during the labelling time of 30-90 minutes. With the concentration of 0-50μg /ml tea polyphenols supplemented in culture

tea polyphenol has no significant effects on the fluidity of L-02 cells membranes. But induced by Fe(Ⅱ)M2O2

fiuorescence polarization increased and the fluidity of L-02 cells membranes decreased. Aner adding the concentration of 10

20

50μg /ml tea polyphenols to the cell suspension respectivily

the fluorescence polarization decreased. The result indicates tea polyphenol could protect damage by reactive oxygen species.