Gel-Extraction for Protein Concentration and Process Optimization

王世昌; 解利昕; 金雷; 余国琮

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Gel-Extraction for Protein Concentration and Process Optimization

更新时间：2025-12-16

- Gel-Extraction for Protein Concentration and Process Optimization
- CIESC Journal Issue 1, (1990)
- 作者机构：
  
  天津大学化学工程研究所,天津大学化学工程研究所,天津大学化学工程研究所,天津大学化学工程研究所天津,天津,现在国家海洋局天津海水淡化与综合利用研究所工作,,天津,天津
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：25 February 1990，
  
  Published：25 February 1990
- 稿件说明：
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王世昌, 解利昕, 金雷, et al. Gel-Extraction for Protein Concentration and Process Optimization[J]. CIESC Journal, 1990, (1).
DOI：

王世昌, 解利昕, 金雷, et al. Gel-Extraction for Protein Concentration and Process Optimization[J]. CIESC Journal, 1990, (1). DOI：

摘要

研究了淀粉—聚丙烯酰胺接枝共聚物等三种凝胶吸水溶胀和在某一酸度条件下收缩释水的过程.利用凝胶这一酸敏效应可以构成萃取循环.以浓缩溶液中的蛋白质或其他大分子物质.文中还讨论了溶液中离子种类、溶质浓度和凝胶粒度等对其溶胀、收缩性能的影响.在对实验数据进行回归分析的基础上

建立了按复合形法进行计算的优化方法

得出实际分离过程的最佳溶胀—收缩范围.

Abstract

The polymer gels of H-SPAM

PAA-Na and S-PAA-Na can swell in water and collapse suddenly at a given pH

with the water released. By using them as extractants

a new process for protein concentration has been developed. The properties of acid-sensitive gel

the influences of ions

solute concentration and gel size on the performance of gel-extraction were studied experi-mentally.Optimization of the acid-sensitive gel-extraction process with complex method has been developed on the basis of regression oi experimental data.

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