Liquid-Liquid Extraction of Proteins by Using AOT-Phospholipid Reverse Micelle System

史红勤; 雷夏; 沈忠耀

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Liquid-Liquid Extraction of Proteins by Using AOT-Phospholipid Reverse Micelle System

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- Liquid-Liquid Extraction of Proteins by Using AOT-Phospholipid Reverse Micelle System
- CIESC Journal Issue 6, (1990)
- 作者机构：
  
  清华大学化工系,清华大学化工系,清华大学化工系 ,北京,北京,北京
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：25 December 1990，
  
  Published：25 December 1990
- 稿件说明：
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史红勤, 雷夏, 沈忠耀. Liquid-Liquid Extraction of Proteins by Using AOT-Phospholipid Reverse Micelle System[J]. CIESC Journal, 1990, (6).
DOI：

史红勤, 雷夏, 沈忠耀. Liquid-Liquid Extraction of Proteins by Using AOT-Phospholipid Reverse Micelle System[J]. CIESC Journal, 1990, (6). DOI：

摘要

研究了PC/AOT和PE/AOT混合体系在有机溶剂中形成反胶团的性质及其萃取蛋白质的性能

并解决了反胶团萃取中有磷脂存在时的蛋白质浓度的分析问题.研究结果表明

在AOT体系中加入磷脂能使胶团尺寸变大

且加入PE能提高血红蛋白和枯草杆菌a—淀粉酶的萃取率

加入PC则常使萃取性能变差.因此

在反胶团萃取蛋白质时

影响萃取的主要因素不仅是胶团的大小

还有胶团与蛋白质之间的相互作用.

Abstract

The water contents (Wo) of reverse micelles formed in a polar solvents by the addition of phosphatidylcholine(PC)

phosphtidylethanolamine(PE)and the mixed system PC/AOT and PE/AOT have been studied. In a reverse micelle system Wo reflects the micelle size. The performance of extraction of proteins by these system has also been investigated. An assay was developed to determine the protein concentration in apolar phase in the presence of PC or PE. It was shown that the reverse micelles formed by PC or PE could not extract lysozyme

ribonuclease-a and cytochrome-c. The results of Wo measurement indicated that adding PC or PE to AOT reverse micell system made the reverse micelles much larger. Adding PE to AOT reverse micelle system increased the solubilities of some proteins in apolar phase

while adding PC to AOT system usually decreased the solubilities. It seems that the main factor affecting the extraction of proteins by reverse micelles is not the size of reverse micelle

but the interaction between the micelle and the proteins.

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