Determination of adsorption of ginsenoside Rg<sub>1</sub> and Re by frontal analysis chromatography

WU Xiuhong; LIN Bingchang

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Determination of adsorption of ginsenoside Rg₁ and Re by frontal analysis chromatography

更新时间：2025-12-10

- Determination of adsorption of ginsenoside Rg₁ and Re by frontal analysis chromatography
- CIESC Journal Issue 9, Pages: 2270-2275(2007)
- 作者机构：
  
  1. 大连理工大学化工学院
  2. 鞍山科技大学分离技术中心
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：05 September 2007，
  
  Published：05 September 2007
- 稿件说明：
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WU Xiuhong, LIN Bingchang. Determination of adsorption of ginsenoside Rg₁ and Re by frontal analysis chromatography[J]. CIESC Journal, 2007, (9): 2270-2275.
DOI：

WU Xiuhong, LIN Bingchang. Determination of adsorption of ginsenoside Rg₁ and Re by frontal analysis chromatography[J]. CIESC Journal, 2007, (9): 2270-2275. DOI：

摘要

制备色谱分离条件优化的基础是吸附等温线的测定及保留时间的预测。本文以人参皂甙Rg1、Re为研究对象，用色谱单柱制备了人参粗粉中的人参皂甙Rg1、Re

以迎头法测定了人参皂甙Rg1、Re在C18球形固定相中的吸附等温线，在竞争Langmuir模型基础上确定了吸附参数G和b，并应用非线性色谱理论的结果预测了人参皂甙Re的保留时间，上述结果与实验值进行了比较，平均相对误差为3.68%。在实验中发现了运用竞争Langmuir模型进行拟合的一些问题并进行了讨论。

Abstract

The determination of isotherm and prediction of retention values are the basis of the optimization of separation conditions of preparative chromatography.In this paper

the isotherms of ginsenoside Rg1 and Re from Panax ginseng were studied.Ginsenoside Rg1 and Re were prepared by chromatography column from Panax ginseng raw material

and the isotherms of ginsenoside Rg1 and Re were determined by frontal analysis (FA) on spherical C18 solid phase.The adsorption parameters G and b were set based on the competitive Langmuir model .In the mean time

the retention time of ginsenoside Re was obtained with the results of nonlinear chromatography theory.The calculated retention time were compared with that of experimental results with average relative error 3.68%.Additionally

some problems related to fitting based on the competitive Langmuir model were found and discussed.

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Determination of adsorption of ginsenoside Rg1 and Re by frontal analysis chromatography

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Determination of adsorption of ginsenoside Rg₁ and Re by frontal analysis chromatography