Improvement of 1,3-propanediol production by recombinant formate dehydrogenase in Klebsiella pneumoniae

黄志华 刘铭 张延平 曹竹安

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Improvement of 1,3-propanediol production by recombinant formate dehydrogenase in Klebsiella pneumoniae

更新时间：2025-12-10

- Improvement of 1,3-propanediol production by recombinant formate dehydrogenase in Klebsiella pneumoniae
- CIESC Journal Issue 4, (2007)
- 作者机构：
  
  1. 清华大学化学工程系生物化工研究所
  2. 三明学院化学与生物工程系
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：05 April 2007，
  
  Published：05 April 2007
- 稿件说明：
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黄志华刘铭张延平曹竹安. Improvement of 1,3-propanediol production by recombinant formate dehydrogenase in Klebsiella pneumoniae[J]. CIESC Journal, 2007, (4).
DOI：

黄志华刘铭张延平曹竹安. Improvement of 1,3-propanediol production by recombinant formate dehydrogenase in Klebsiella pneumoniae[J]. CIESC Journal, 2007, (4). DOI：

摘要

在甘油厌氧发酵生产1

3-丙二醇的过程中，需要消耗还原当量NADH，NADH的有效供给决定了1

3-丙二醇的产量。本文从Candida boidinii基因组DNA中克隆了甲酸脱氢酶基因fdh，利用表达质粒pMAL TM-p2X-fdh转化到1

3-丙二醇生产菌 Klebsiella pneumoniae YMU2中，构建了具有NADH再生系统的重组菌Klebsiella pneumoniae F-1。在5 L发酵罐培养中，F-1合成1

3-丙二醇浓度和产率分别达到了78. 6 g·L-1 和1. 33 g·L-1·h-1，分别比YMU2提高了12. 5% 和41. 2%。根据F-1和YMU2菌株的主要代谢产物的生成情况比较了二者的代谢流分布。

Abstract

Biosynthesis of 1

3-propanediol from glycerol by Klebsiella pneumoniae was associated with the consumption of NADH

which was generated/regenerated in the oxidative branch pathway of glycerol in vivo. The availability of NADH would be critical for the final yield of 1

3-propanediol. Formate dehydrogenase gene(fdh) was cloned from Candida boidinii genome and was then transformed into Klebsiella pneumoniae YMU2 by constructed expression vector pMAL TM-p2X-fdh to generate recombinant Klebsiella pneumoniae F-1. Compared with K. pneumoniae YMU2

the yield of 1

3-propanediol and production rate of recombinant F-1 were 78. 6 g·L-1 and 1. 33 g·L-1·h-1

increased by 12. 5% and 41. 2%

respectively. The metabolic flux of strain YMU2 and recombinant F-1 was analyzed with the formation of main metabolites.

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