一株对苯二甲酸降解菌的鉴定及其降解特性

FENG Yangyang; CHEN Jun; LIU Bo; CHEN Yingwen; ZHENG Guoyang; LU Jianhua; SHEN Shubao

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一株对苯二甲酸降解菌的鉴定及其降解特性

更新时间：2025-12-10

- 一株对苯二甲酸降解菌的鉴定及其降解特性
- CIESC Journal Issue 8, (2006)
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- Online First：25 August 2006，
  
  Published：25 August 2006
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FENG Yangyang, CHEN Jun, LIU Bo, et al. 一株对苯二甲酸降解菌的鉴定及其降解特性[J]. CIESC Journal, 2006, (8).
DOI：

FENG Yangyang, CHEN Jun, LIU Bo, et al. 一株对苯二甲酸降解菌的鉴定及其降解特性[J]. CIESC Journal, 2006, (8). DOI：

摘要

从扬子石油化工责任有限公司废水处理装置的污泥池中分离、筛选得到了一株高效降解对苯二甲酸（PTA）的菌株，PA-18.采用PCR扩增获得该菌16SrDNA片段，核苷酸序列分析结果表明：该菌株的16SrDNA的核苷酸序列与多株假单胞菌（Pseudomonas sp.）的同源性为99%，在细菌系统发育分类学上，该菌株属于假单胞属.在温度为37℃、pH 7.0、OD660 nm2.0条件下，反应24 h，此菌对PTA的降解率大于95 %.同时对于实际的PTA废水也有很好的处理效果，CODCr去除率高达85%.在好氧降解作用下，PTA降解的主要中间物被确定为原儿茶酸.

Abstract

A strain PA-18 was isolated from the active sludge of p-phthalic acid (PTA) wastewater treatment in Yangtze Petrochemical Company.The 16SrDNA fragment was amplified from strain PA-18 by PCR，and the 16SrDNA sequence analysis indicated that strain PA-18 shared 99% 16SrDNA sequence homology with Pseudomonas sp..In the phylogenetic framework of bacterial classification，PA-18 belongs to Pseudomonas sp..Under the condition of temperature 37℃，pH 7.0 and OD660 nm2.0，the PTA decomposition rate of this bacterial strain was beyond 95% after 24 h.Meanwhile，this strain also could treat PTA wastewater with CODCr removal of 85%.p-Phthalic acid was degraded by the strain PA-18 through the intermediate formation of protocatechuic acid in the aerobic state.

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