Purification and structural characterization of ε-PL produced by Kitasatospora sp.PL6-3

ZHOU Jun; XU Hong; WANG Jun; YAO Zhong; WANG Hui; OUYANG Pingkai

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Purification and structural characterization of ε-PL produced by Kitasatospora sp.PL6-3

更新时间：2025-12-10

- Purification and structural characterization of ε-PL produced by Kitasatospora sp.PL6-3
- CIESC Journal Issue 8, (2006)
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- Online First：25 August 2006，
  
  Published：25 August 2006
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ZHOU Jun, XU Hong, WANG Jun, et al. Purification and structural characterization of ε-PL produced by Kitasatospora sp.PL6-3[J]. CIESC Journal, 2006, (8).
DOI：

ZHOU Jun, XU Hong, WANG Jun, et al. Purification and structural characterization of ε-PL produced by Kitasatospora sp.PL6-3[J]. CIESC Journal, 2006, (8). DOI：

摘要

从北里孢菌（Kitasatospora sp.）PL6-3的发酵液中分离纯化ε-聚赖氨酸（ε-PL），首先发酵液经离子交换，得到ε-PL粗品，然后通过凝胶过滤Sephadex G-25柱层析，获得了色谱纯的产品，分离总收率为81.2%.利用紫外吸收光谱、红外光谱（IR）和高分辨率魔角旋转核磁共振谱（HR/MAS NMR），确认了该聚合物的结构.用SDS-PAGE电泳和凝胶渗透色谱（GPC）测得ε-PL的相对分子质量分别为5.01×103和5.05×103，略高于文献报道的其他菌所产的ε-PL.

Abstract

ε-Poly-L-lysine(ε-PL) was isolated and purified from the culture broth of Kitasatospora sp.PL6-3.Through the ion exchange process and Sephadex G-25 gel filtration chromatography

a high purity product was obtained with overall yield of 81.2%.The purified ε-PL was characterized with UV

IR and HR/MAS NMR.Its relative molecular weights as determined by SDS-PAGE and gel permeation chromatography (GPC) were about 5.01×103 and 5.05×103 respectively

which was higher than the other ε-PL reported before.

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