两相系统中不对称还原2-辛酮的菌株筛选

HU Jian; XU Yan

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两相系统中不对称还原2-辛酮的菌株筛选

更新时间：2025-12-10

- 两相系统中不对称还原2-辛酮的菌株筛选
- CIESC Journal Issue 10, (2006)
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- Online First：25 October 2006，
  
  Published：25 October 2006
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HU Jian, XU Yan. 两相系统中不对称还原2-辛酮的菌株筛选[J]. CIESC Journal, 2006, (10).
DOI：

HU Jian, XU Yan. 两相系统中不对称还原2-辛酮的菌株筛选[J]. CIESC Journal, 2006, (10). DOI：

摘要

从93株酵母、霉菌和细菌中筛选出能不对称还原2-辛酮的菌株.经过筛选获得一株博伊丁假丝酵母Candida boidinii CECT 10035.该菌种在底物浓度为78 mmol·L-1时，能转化得到S-2-辛醇，2-辛酮转化率为32.6%，e.e.值达到95.6%.同时，还筛选出一株酒明串珠菌Oenococcus oeni CECT 4730，可以转化得到R-2-辛醇，当底物浓度为78 mmol·L-1时，2-辛酮转化率为99.8%，e.e.值为97.8%.

Abstract

Asymmetric reduction of 2-octanone by yeast，mold and bacteria in aqueous-organic solvents biphasic systems were investigated.Candida boidinii CECT 10035 catalyzed the reduction of 2-octanone to S-2-octanol with conversion of 32.6% and optical purity of 95.6%.Different from the yeast strain， Oenococcus oeni CECT 4730 catalyzed the reduction of 2-octanone to R-2-octanol with higher activity and optical purity.When the concentration of 2-octanone was 78 mmol·L-1，the conversion and enantiomeric excess were 99.8% and 97.8%.

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