PURIFICATION AND IDENTIFICATION OF NOVEL BIOFLOCCULANT

HE Ning; LI Yin; CHEN Jian; LUN Shiyi

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PURIFICATION AND IDENTIFICATION OF NOVEL BIOFLOCCULANT

更新时间：2025-12-10

- PURIFICATION AND IDENTIFICATION OF NOVEL BIOFLOCCULANT
- CIESC Journal Issue 10, (2002)
- 作者机构：
  
  无锡轻工大学生物工程学院环境生物技术研究室
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：25 October 2002，
  
  Published：25 October 2002
- 稿件说明：
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HE Ning, LI Yin, CHEN Jian, et al. PURIFICATION AND IDENTIFICATION OF NOVEL BIOFLOCCULANT[J]. CIESC Journal, 2002, (10).
DOI：

HE Ning, LI Yin, CHEN Jian, et al. PURIFICATION AND IDENTIFICATION OF NOVEL BIOFLOCCULANT[J]. CIESC Journal, 2002, (10). DOI：

摘要

从土壤中筛选获得了一株絮凝剂产生菌

经初步鉴定为诺卡氏菌 (Nocardiasp .) .考察了该菌株的基本生长代谢特性

其合成的絮凝剂约 80 %以上分泌至胞外培养基中 .利用有机溶剂沉淀结合柱层析法从Nocardiasp .CCTCCM 2 0 10 0 5的发酵液中提取到一种新型生物絮凝剂REA - 11.分析表明

REA - 11是一种以半乳糖醛酸为主要结构单元的酸性蛋白聚糖

Abstract

This paper mainly discussed a flocculant-producing bacteria isolated from soil and the flocculating substance secreted into the medium.Preliminary studies identified the bacteria CCTCC M201005 as Nocardia species.Its growth and metabolism were studied.More than 80% of the flocculating activity was detected in the culture broth.A new bioflocculant--REA-11 was purified by repeated ethanol precipitation

ion exchange and gel chromatography from the culture broth.Its molecular mass was estimated to be over 10 5g·mol -1 by the gel filtration method.The results of investigation on the purified REA-11 and its acid hydrolysate by colcrimetric analysis

paper chromatography

HPLC and GC-MS suggested that REA-11 was a novel acidic polysaccharide mainly composed of galactocuronic acid with little acetic acid but no hexosamine detected.Protein was not a necessary part for the flocculating activity of REA-11

but mainly assisting in connecting the monosaccharides in the macromolecule or maintaining the dimensional conformation of the active biopolymer.

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