PRODUCTION OF POLY-β-HYDROXYBUTYRATE BY FED-BATCH CULTURE OF NOVEL RECOMBINANT Escherichia coli VG1(pTU14)

YU Huimin; ZHANG Yanping; SHI Yue; YANG Shengli; SHEN Zhongyao

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PRODUCTION OF POLY-β-HYDROXYBUTYRATE BY FED-BATCH CULTURE OF NOVEL RECOMBINANT Escherichia coli VG1(pTU14)

更新时间：2025-12-10

- PRODUCTION OF POLY-β-HYDROXYBUTYRATE BY FED-BATCH CULTURE OF NOVEL RECOMBINANT Escherichia coli VG1(pTU14)
- CIESC Journal Issue 7, (2002)
- 作者机构：
  
  1. 清华大学化学工程系
  2. 中国科学院上海生物工程研究中心
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：25 July 2002，
  
  Published：25 July 2002
- 稿件说明：
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YU Huimin, ZHANG Yanping, SHI Yue, et al. PRODUCTION OF POLY-β-HYDROXYBUTYRATE BY FED-BATCH CULTURE OF NOVEL RECOMBINANT Escherichia coli VG1(pTU14)[J]. CIESC Journal, 2002, (7).
DOI：

YU Huimin, ZHANG Yanping, SHI Yue, et al. PRODUCTION OF POLY-β-HYDROXYBUTYRATE BY FED-BATCH CULTURE OF NOVEL RECOMBINANT Escherichia coli VG1(pTU14)[J]. CIESC Journal, 2002, (7). DOI：

摘要

利用已构建的同时含有透明颤菌血红蛋白基因 (vgb)、λ噬菌体裂解基因 (S-RRz)和PHB合成基因(phbCAB)的产PHB基因工程大肠杆菌VG1(pTU14 )

在 5L发酵罐里进行补料分批培养 .研究发现 :碳氮比(C/N)

DO(溶氧 )及碳源和氮源的浓度是影响菌体生长和PHB积累的重要因素 ;补料时间可以通过DO和 pH值两个参数的变化在线综合识别 ;流加策略应使发酵前期菌体大量生长

发酵后期PHB大量积累 .在优化条件下

对VG1(pTU14 )进行 6 0h的补料分批培养

菌体细胞浓度、PHB浓度和PHB占细胞干质的分数分别高达2 15 .9g·L-1、 193.7g·L-1和 89.7% .

Abstract

VG1(pTU14) is a novel recombinant Escherichia coli for Poly- β -hydroxybutyrate (PHB) production

which contains Vitreoscilla hemoglobin gene ( vgb )

lytic genes of phage λ( S -RRz ) and PHB biosynthetic genes ( phb CAB) in the cell.During the process of fed-batch culture of VG1 (pTU14)

it was found that the level of dissolved oxygen (DO)

the concentration ratio (C/N) and the range of carbon and nitrogen source were all important parameters affecting cell growth and PHB accumulation.DO and pH could be used to decide the time for the nutrition feeding.When C/N

carbon and nitrogen concentration were in the range of 10-20

10-40g·L -1

1.0-2.0g·L -1 respectively and DO was higher than 5%

the cell growth was enhanced.Otherwise

PHB accumulation would be enhanced too early to attain the high cell density culture.Finally

the optimal feeding strategy

by which the cell growth was emphasized in early process and the PHB accumulation was promoted in late process

was carried out.After 60h fed-batch culture under the optimal conditions

cell concentration

PHB concentration and PHB content were reached up to 215.9g·L -1

193.7?g·L -1 and 89.7%

respectively.

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