MECHANISM AND KINETIC ANALYSIS OF ENZYMATIC LIMITED HYDROLYSIS OF SOY PROTEIN ISOLATE

LIU Lin; YE Feng; OU Zhimin

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MECHANISM AND KINETIC ANALYSIS OF ENZYMATIC LIMITED HYDROLYSIS OF SOY PROTEIN ISOLATE

更新时间：2025-12-10

- MECHANISM AND KINETIC ANALYSIS OF ENZYMATIC LIMITED HYDROLYSIS OF SOY PROTEIN ISOLATE
- CIESC Journal Issue 2, (2002)
- 作者机构：
  
  浙江工业大学生物与环境工程学院
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：25 February 2002，
  
  Published：25 February 2002
- 稿件说明：
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LIU Lin, YE Feng, OU Zhimin. MECHANISM AND KINETIC ANALYSIS OF ENZYMATIC LIMITED HYDROLYSIS OF SOY PROTEIN ISOLATE[J]. CIESC Journal, 2002, (2).
DOI：

LIU Lin, YE Feng, OU Zhimin. MECHANISM AND KINETIC ANALYSIS OF ENZYMATIC LIMITED HYDROLYSIS OF SOY PROTEIN ISOLATE[J]. CIESC Journal, 2002, (2). DOI：

摘要

Abstract

The enzymatic limited hydrolysis of soy protein isolate (SPI) by Alcalase 0.6L was carried out with pH-stat method at pH 8.0

50℃.The effects of the main parameters on the degree of hydrolysis

were investigated and a model based on the experiment results was deduced.The results showed that the substrate and the enzyme-substrate intermediate induced enzyme inhibition

which occurred due to free enzyme attacking the enzyme-substrate complexes.A critical 0 would correspond to a constant 0

which was 96.77mg·ml -1 under the experiment condition.The kinetic parameters calculated from the experiment data were K m=0.016mol·L -1 and V max =0.765mol·min·L -1 .The results showed that x can be regulated to about 6.0 with the mentioned factors in 30min.The good fitness between predicted and measured results confirmed the usefulness of the model.

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