REFOLDING OF DENATURED-REDUCED LYSOZYME FACILITATED BY β-CYCLODEXTRIN

DONG Xiaoyan; SHI Jinhui; SUN Yan

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REFOLDING OF DENATURED-REDUCED LYSOZYME FACILITATED BY β-CYCLODEXTRIN

更新时间：2025-12-10

- REFOLDING OF DENATURED-REDUCED LYSOZYME FACILITATED BY β-CYCLODEXTRIN
- CIESC Journal Issue 4, (2002)
- 作者机构：
  
  天津大学化工学院生物化工系
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：25 April 2002，
  
  Published：25 April 2002
- 稿件说明：
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DONG Xiaoyan, SHI Jinhui, SUN Yan. REFOLDING OF DENATURED-REDUCED LYSOZYME FACILITATED BY β-CYCLODEXTRIN[J]. CIESC Journal, 2002, (4).
DOI：

DONG Xiaoyan, SHI Jinhui, SUN Yan. REFOLDING OF DENATURED-REDUCED LYSOZYME FACILITATED BY β-CYCLODEXTRIN[J]. CIESC Journal, 2002, (4). DOI：

摘要

研究了 β -环糊精 (β -CD)和表面活性剂十六烷基三甲基溴化铵 (CTAB)作为复性液添加剂对促进变性 -还原溶菌酶的氧化复性 .β -CD自身具有抑制肽链分子间聚集

提高复性率的作用

但其相对于变性酶的浓度需达到 30 0 0～ 5 0 0 0倍以上

否则作用效果不明显 .与其相比

利用CTAB和 β -CD联合作用系统具有更佳的促进复性效果

在适宜的盐酸胍浓度存在下可使较高浓度的变性 -还原酶实现完全复性 .确定了CTAB +β -CD系统促进变性酶复性的最佳操作 pH值和温度

Abstract

The oxidative refolding of denatured-reduced lysozyme in the presence of cetyl trimethyl ammonium bromide (CTAB) and/or β -cyclodextrin ( β -CD) was investigated. The renaturation yield was improved in the presence of β -CD which inhibited the hydrophobic interaction between the unfolded protein molecules. However

β -CD concentration should be 3000-5000 times higher than the denatured enzyme to achieve a significant refolding effect. In contrast to β -CD

the refolding system with both CTAB and β -CD showed more favorable effect on the enzyme renaturation. With a properly enhanced guanidine hydrochloride concentration

the enzymatic activity of the denatured-reduced lysozyme could be completely regained. In addition

the optimal pH (8.5) and temperature (37℃) was established for the CTAB+ β -CD renaturation system.

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