MASS TRANSFER KINETICS IN ADSORPTION PROCESS IN PREPARATIVE ELECTROPHORETIC AFFINITY CHROMATOGRAPHY

FENG Shaohua; LIU Zheng; DING Fuxin; YUAN Naiju

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MASS TRANSFER KINETICS IN ADSORPTION PROCESS IN PREPARATIVE ELECTROPHORETIC AFFINITY CHROMATOGRAPHY

更新时间：2025-12-10

- MASS TRANSFER KINETICS IN ADSORPTION PROCESS IN PREPARATIVE ELECTROPHORETIC AFFINITY CHROMATOGRAPHY
- CIESC Journal Issue 9, (2001)
- 作者机构：
  
  清华大学化学工程系
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：25 September 2001，
  
  Published：25 September 2001
- 稿件说明：
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FENG Shaohua, LIU Zheng, DING Fuxin, et al. MASS TRANSFER KINETICS IN ADSORPTION PROCESS IN PREPARATIVE ELECTROPHORETIC AFFINITY CHROMATOGRAPHY[J]. CIESC Journal, 2001, (9).
DOI：

FENG Shaohua, LIU Zheng, DING Fuxin, et al. MASS TRANSFER KINETICS IN ADSORPTION PROCESS IN PREPARATIVE ELECTROPHORETIC AFFINITY CHROMATOGRAPHY[J]. CIESC Journal, 2001, (9). DOI：

摘要

建立了描述电动亲和色谱分离过程吸附操作的传质方程

研究了人血清白蛋白 (HSA)和BlueSepharoseFastFlow (Blue)体系中Blue介质的电渗特性及其影响因素 ;测定了Blue对HSA的吸附等温线以及在不同交变电场下的动态吸附曲线 ;由模型计算得到了吸附操作的传质系数及其与电场强度和交变频率的关系

实验结果和计算结果均表明

施加交变电场有助于提高传质系数

从而强化吸附过程的传质 .实验结果揭示了电渗流在强化色谱介质颗粒内传质的重要作用及其在发展大规模电动色谱技术中的良好应用前景

Abstract

A correlation for calculating the mass transfer coefficient of the adsorption process in the electrophoretic affinity chromatography (EAC) was established. Adsorption of human serum albumin (HSA) on Blue Sepharose Fast Flow(Blue)in alternating electric field was conducted at different electric field strength and alternating frequency to illustrate the effects of electroosmosis on the adsorption process of the EAC. The experimental results showed that the increase in current density led to corresponding increases in both electrosmotic flux and dynamic binding capacity. The mass transfer coefficient was obtained as a function of electric field strength

as indicated by current density

and alternating frequency. The occurrence of electrosmotic flux inside the porous gel particle greatly increased the speed of the migration of biological molecules towards the ligand immobilized in the inner surface of the particle and consequently resulted in an improved adsorption behavior

as shown by the increase in mass transfer coefficient. The results described in the present paper demonstrated the high potential of electroosmosis in the development of large scale electrophoretic chromatography of biological molecules.

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