PROTECTION OF IMMOBILIZED CELLULASE BY TREHALOSE

杨基础; 董燊; 杨小民

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PROTECTION OF IMMOBILIZED CELLULASE BY TREHALOSE

更新时间：2025-12-16

- PROTECTION OF IMMOBILIZED CELLULASE BY TREHALOSE
- CIESC Journal Issue 2, (2000)
- 作者机构：
  
  清华大学化学工程系!北京100084,清华大学化学工程系!北京100084,清华大学化学工程系,北京,100084
- 作者简介：
- 基金信息：
- DOI：
  CLC： 629
- Online First：25 April 2000，
  
  Published：25 April 2000
- 稿件说明：
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杨基础, 董燊, 杨小民. PROTECTION OF IMMOBILIZED CELLULASE BY TREHALOSE[J]. CIESC Journal, 2000, (2).
DOI：

杨基础, 董燊, 杨小民. PROTECTION OF IMMOBILIZED CELLULASE BY TREHALOSE[J]. CIESC Journal, 2000, (2). DOI：

摘要

研究了海藻糖对固定化纤维素酶在干燥和存放过程中的保护作用

发现海藻糖能有效地减少固定化酶干燥过程中酶的热失活

而且能提高固定化纤维素酶存放过程中的热稳定性 .但温度越高海藻糖的保护效果越差 .借助红外分析和差示扫描量热方法

初步推测了海藻糖对固定化纤维素酶保护的机理为 :一是糖的羟基同酶分子以氢键的形式结合

提高了酶的热变性温度 ;二是糖分子包裹在酶分子周围

或填充在酶分子的空间结构内

特别是酶的活性部位附近

并形成玻璃态

将酶蛋白的空间结构固定住而避免酶的失活 .

Abstract

The protection of immobilized cellulase by trehalose during drying and storage is investigated. It is found that trehalose can reduce the activity losts of immobilized cellulase efficiently during its drying

and the mass ratio of trehalose and enzyme is about 0.25 at 58 ℃. The stability of immobilized cellulase increases with increase of trehalose or decrease of storage temperature. By mean of infrared spectrometry and DSC

the mechanismof protection of immobilized cellulase by trehalose is studied. Increase of stability of immobilized enzymes is not only because of the interaction between trehalose and enzyme

but also because of the formation of a glassy state in and around enzyme moleculars

especially near active sites of enzymes

so that the structure of enzymes is restricted

and activities of enzyme can be preserved at a high temperature.

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