SEPARATION OF GLUTATHIONE BY AQUEOUS TWO-PHASE EXTRACTION COMBINED WITH TEMPERATURE-INDUCED PHASE SEPARATION

Mei lehe; Lin Dongqiang; Zhu Ziqiang(Dept.of Biochem.Eng.

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SEPARATION OF GLUTATHIONE BY AQUEOUS TWO-PHASE EXTRACTION COMBINED WITH TEMPERATURE-INDUCED PHASE SEPARATION

更新时间：2025-12-16

- SEPARATION OF GLUTATHIONE BY AQUEOUS TWO-PHASE EXTRACTION COMBINED WITH TEMPERATURE-INDUCED PHASE SEPARATION
- CIESC Journal Issue 4, (1998)
- 作者机构：
  
  浙江大学生物化工系!杭州310027,浙江大学生物化工系!杭州310027,浙江大学生物化工系,杭州,310027
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q028
- Online First：25 August 1998，
  
  Published：25 August 1998
- 稿件说明：
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Mei lehe, Lin Dongqiang, Zhu Ziqiang(Dept.of Biochem.Eng.. SEPARATION OF GLUTATHIONE BY AQUEOUS TWO-PHASE EXTRACTION COMBINED WITH TEMPERATURE-INDUCED PHASE SEPARATION[J]. CIESC Journal, 1998, (4).
DOI：

Mei lehe, Lin Dongqiang, Zhu Ziqiang(Dept.of Biochem.Eng.. SEPARATION OF GLUTATHIONE BY AQUEOUS TWO-PHASE EXTRACTION COMBINED WITH TEMPERATURE-INDUCED PHASE SEPARATION[J]. CIESC Journal, 1998, (4). DOI：

摘要

对用双水相分配技术结合温度诱导相分离技术从酵母中提取谷胱甘肽（GSH）进行了研究．考察了GSH在环氧乙烷──环氧丙烷无规共聚物（EOPO）／羟丙基淀粉（PES）系统中的分配行为，包括两次分离过程（双水相分配及温度诱导过程)中的不同系统组成、pH等对GSH分配的影响，确定了较佳的双水相系统──EOPO400013％，PES10010％，pH=10.5．在此基础上进行了从酵母细胞中提取GSH的工艺研究，设计了合理的分离流程，研究结果表明GSH的总萃取率可达80％以上．

Abstract

The techniques for separation of glutathione（GSH）by aqueous two-phase extraction combined with temperature-induced phase separation were studied.The partition behavior of GSH in ethylene oxide-propylene oxide random copolymer(EOPO）/hydroxypropyl starch（PES)／water systems was investigated．The effects of such factors

as concentration of polymer

on the partition coefficients in two separation processes（aqueous two-phase extraction and temperature-induced phase separation)were studied.The optimum conditions were EOPO4000 13%

PES100 10％

pH=10.5. The separation technique for GSH from yeast cell was also investigated.A feasible separation scheme was suggested

and the results show that the total extraction yield could reach over 80％．

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