TRYPSIN PURIFICATION BY AFFINITY PRECIPITATION WITH POLYMERIZED LIPOSOME AS LIGAND CARRIER

Dong Xiaoyan; Sun Yan; Yu Kai; Jin Xianhua(Department of Chemical Engineering

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TRYPSIN PURIFICATION BY AFFINITY PRECIPITATION WITH POLYMERIZED LIPOSOME AS LIGAND CARRIER

更新时间：2025-12-16

- TRYPSIN PURIFICATION BY AFFINITY PRECIPITATION WITH POLYMERIZED LIPOSOME AS LIGAND CARRIER
- CIESC Journal Issue 2, (1996)
- 作者机构：
  
  天津大学化学工程系!天津300072,天津大学化学工程系!天津300072,天津大学化学工程系!天津300072,天津大学化学工程系,天津,300072
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q028
- Online First：25 April 1996，
  
  Published：25 April 1996
- 稿件说明：
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Dong Xiaoyan, Sun Yan, Yu Kai, et al. TRYPSIN PURIFICATION BY AFFINITY PRECIPITATION WITH POLYMERIZED LIPOSOME AS LIGAND CARRIER[J]. CIESC Journal, 1996, (2).
DOI：

Dong Xiaoyan, Sun Yan, Yu Kai, et al. TRYPSIN PURIFICATION BY AFFINITY PRECIPITATION WITH POLYMERIZED LIPOSOME AS LIGAND CARRIER[J]. CIESC Journal, 1996, (2). DOI：

摘要

Abstract

A polymerized liposome (PLS) was prepared using a synthesized phospholipid with a diacetylene moiety.The precipitable property of the PLS on salt additions

ligand immobilization

affinity adsorption kinetics and adsorption isotherms were studied.Trypsin was purified by affinity precipitation from a crude pancreatic extract.The specific activity reached about 13000 U.mg~(－1). These results indicate that the PLS is a potential ligand carrier for affinity precipitation of proteins.

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