STUDY ON SEPARATION DEVICE AND PROCESS THEORY OF COUNTERACTING CHROMATOGRAPHIC　ELECTROPHORESIS

高瑞昶; 王世昌

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STUDY ON SEPARATION DEVICE AND PROCESS THEORY OF COUNTERACTING CHROMATOGRAPHIC　ELECTROPHORESIS

更新时间：2025-12-16

- STUDY ON SEPARATION DEVICE AND PROCESS THEORY OF COUNTERACTING CHROMATOGRAPHIC　ELECTROPHORESIS
- CIESC Journal Issue 3, (1995)
- 作者机构：
  
  天津大学化学工程研究所!天津300072,天津大学化学工程研究所,天津,300072
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q028
- Online First：25 June 1995，
  
  Published：25 June 1995
- 稿件说明：
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高瑞昶, 王世昌. STUDY ON SEPARATION DEVICE AND PROCESS THEORY OF COUNTERACTING CHROMATOGRAPHIC　ELECTROPHORESIS[J]. CIESC Journal, 1995, (3).
DOI：

高瑞昶, 王世昌. STUDY ON SEPARATION DEVICE AND PROCESS THEORY OF COUNTERACTING CHROMATOGRAPHIC　ELECTROPHORESIS[J]. CIESC Journal, 1995, (3). DOI：

摘要

研制成功凝胶色谱电泳(CACE)分离装置

采用标准蛋白质模式混合物进行实验研究。通过理论分析

用体积平均方法建立了过程数学模型

计算并比较了不同条件下目标蛋白质的聚焦过程及分离操作的动态特性

说明了流场与电场强度对分离过程的重要影响。模拟结果与实验数据充分吻合。该模型对CACE分离过程及装置的设计和优化提供了理论依据。

Abstract

Counteracting chromatgraphic electrophoresis (CAGE)

a preparative separation device is successfully constructed and tested. Experiments are performed with protein mixtures of BSA

Mg and Cyto - c to study the separation performances

concentration profiles

start -up ways as well as influences of pH and ionic strength on separation process. Mathematical models are proposed based on the fluid dynamics of porous media. Results indicate that the CACE process has a good potential in protein and fine chemicals purification.

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