A STUDY OF CELL DEBRIS REMOVAL BY POLYETHYLENE GLYCOL PRECIPITATION

Xiu Zhilong; Jiang Wei and Su Zhiguo(Coll. of Chem. Eng. Dalian University of Technology; Dalian 116012)

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A STUDY OF CELL DEBRIS REMOVAL BY POLYETHYLENE GLYCOL PRECIPITATION

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- A STUDY OF CELL DEBRIS REMOVAL BY POLYETHYLENE GLYCOL PRECIPITATION
- CIESC Journal Issue 6, (1993)
- 作者机构：
  
  大连理工大学化工学院,大连理工大学化工学院,大连理工大学化工学院大连116012,大连116012,大连,116012
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：25 December 1993，
  
  Published：25 December 1993
- 稿件说明：
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Xiu Zhilong, Jiang Wei and Su Zhiguo(Coll. of Chem. Eng. Dalian University of Technology, Dalian 116012). A STUDY OF CELL DEBRIS REMOVAL BY POLYETHYLENE GLYCOL PRECIPITATION[J]. CIESC Journal, 1993, (6).
DOI：

Xiu Zhilong, Jiang Wei and Su Zhiguo(Coll. of Chem. Eng. Dalian University of Technology, Dalian 116012). A STUDY OF CELL DEBRIS REMOVAL BY POLYETHYLENE GLYCOL PRECIPITATION[J]. CIESC Journal, 1993, (6). DOI：

摘要

自 80年代以来，双水相苯取技术用于生化产品的分离受到重视，尤其对聚乙二醇（PEG）和无机盐体系的开发研究更多“，“，双水相荤取技术在分离细胞匀浆中胞内产物和细胞碎片方面，显示出可取代高速离心和微孔膜过滤的潜力。目前大多数研究集中在双水相革取的机理和分离纯化不同蛋白质上，对清除细胞碎片的应用研究尚不多见。本文从细胞碎片在 PEG－无机盐系统的分配规律中发规系统未形成双水相前，存在一个液－固两相生成区。在此区内大部分碎片形成固相沉淀，低速离心即可除去。以从酵母中提取醇脱氢酶为例，将这种新的售脸碰计茉D体同Nil。t。十签人（门的W＊栅茉阶体淋杆了计林

Abstract

The removal of yeast cell debris following high - speed bead mill disruption was studied by using polyethylene glycol (PEG) precipitation. The partition rule of cell debris was investigated in PEG/inorganic salts systems. Cell debris might be partitioned between the bottom

intermediate and top phases in different aqueous two - phase compositions. When there was only PEG or there were PEG and a little amount of salt

yeast homogenate was separated into a supernatant containing intracellular products and a solid cell debris sediment by using only low speed centrifugation. The effective removal of cell debris and the extraction of intracellular alcohol dehydrogenase were further studied by using the liquid -solid two -phase system and aqueous two -phase systems.

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