PREPARATION AND CHARACTERIZATION OFAFFINITY-LIGAND-MODIFIED LIPOSOMESFOR TRYPSIN SEPARATION

孙彦; 徐晓燕; 王绍亭

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PREPARATION AND CHARACTERIZATION OFAFFINITY-LIGAND-MODIFIED LIPOSOMESFOR TRYPSIN SEPARATION

更新时间：2025-12-16

- PREPARATION AND CHARACTERIZATION OFAFFINITY-LIGAND-MODIFIED LIPOSOMESFOR TRYPSIN SEPARATION
- CIESC Journal Issue 3, (1993)
- 作者机构：
  
  天津大学化学工程系,天津大学化学工程系,天津大学化学工程系天津300072,天津300072,天津,300072
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：25 June 1993，
  
  Published：25 June 1993
- 稿件说明：
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孙彦, 徐晓燕, 王绍亭. PREPARATION AND CHARACTERIZATION OFAFFINITY-LIGAND-MODIFIED LIPOSOMESFOR TRYPSIN SEPARATION[J]. CIESC Journal, 1993, (3).
DOI：

孙彦, 徐晓燕, 王绍亭. PREPARATION AND CHARACTERIZATION OFAFFINITY-LIGAND-MODIFIED LIPOSOMESFOR TRYPSIN SEPARATION[J]. CIESC Journal, 1993, (3). DOI：

摘要

研究了脂质体的制备及利用对氨基苄脒(PAB)修饰的脂质体亲和分离胰蛋白酶的特性.PAB修饰的脂质体与酶结合的平衡常数为游离PAB与酶结合的平衡常数的1/4以上

可有效地用于胰蛋白酶的亲和分离.

Abstract

The preparation and characterization of the affinity-ligand-modified liposomes were studied. The optimum conditions for liposome preparation and the ligand-modifying reactions were determined. Using p-aminobenzamidine (PAB) as an affinity ligand

the trypsin inhibition kinetics by PAB-modified liposomes and the characteristics of affinity ultrafiltration of trypsin were investigated. The results showed that the equilibrium binding constant of the PAB-modified liposomes for trypsin was more than 1/4 of that of free PAB for trypsin and the affinity-ligand-modified liposomes were effective in affinity separation for trypsin.

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