CYLINDRICAL CENTRIFUGAL EXTRACTOR FOR EXTRACTION PURIFICATION OF α-AMYLASE IN AQUEOUS TWO-PHASE SYSTEMS

冯幼琴; 梅乐和; 朱自强; 陈孝国

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CYLINDRICAL CENTRIFUGAL EXTRACTOR FOR EXTRACTION PURIFICATION OF α-AMYLASE IN AQUEOUS TWO-PHASE SYSTEMS

更新时间：2025-12-16

- CYLINDRICAL CENTRIFUGAL EXTRACTOR FOR EXTRACTION PURIFICATION OF α-AMYLASE IN AQUEOUS TWO-PHASE SYSTEMS
- CIESC Journal Issue 5, (1992)
- 作者机构：
  
  浙江大学化工系,浙江大学化工系,浙江大学化工系,浙江大学化工系杭州210027,杭州210027,杭州210027,杭州,210027
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：25 October 1992，
  
  Published：25 October 1992
- 稿件说明：
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冯幼琴, 梅乐和, 朱自强, et al. CYLINDRICAL CENTRIFUGAL EXTRACTOR FOR EXTRACTION PURIFICATION OF α-AMYLASE IN AQUEOUS TWO-PHASE SYSTEMS[J]. CIESC Journal, 1992, (5).
DOI：

冯幼琴, 梅乐和, 朱自强, et al. CYLINDRICAL CENTRIFUGAL EXTRACTOR FOR EXTRACTION PURIFICATION OF α-AMYLASE IN AQUEOUS TWO-PHASE SYSTEMS[J]. CIESC Journal, 1992, (5). DOI：

摘要

根据双水相体系的特点

选择了Ф20(环)圆筒式离心萃取器进行a-淀粉酶的双水相体系萃取实验

考察了萃取器的水力学特性

确定了萃取操作范围

并考察了萃取器转鼓转速、总流量对体系传质特性、a-淀粉酶失活和收率的影响.结果表明

在转速3380～3800r/min、流比0.2～0.5、总流量25～50ml/min范围内

萃取器的轻相夹带量可在10%以内

重相夹带量趋近于零

a-淀粉酶的失活可以忽略不计

萃取效率和收率分别达90%和75%以上.

Abstract

The technical aspects of a new and powerful separation technique

extractive purification by aqueous two-phase systems were studied in detail. According to the characteristics of aqueous two phase systems

a φ20 cylindrical centrifugal extractor was selected as the equipment for extractive purification of α-amylase. The hydraulic characteristics of the installation and the effects of speed and total throughput on the yield and Murphree efficiency of α-amylase extraction were investigated. A speed of 3380-3800r/min

a flow ratio of 0.2-0.5 and a total throughput of 25-50ml/min were found as the optimal operation range for while 90% of phases purity

75% yield and 90% Murphree efficiency for a-amylase could be obtained.

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