Biosynthesis of glycyrrhetinic acid monoglucuronide by whole-cell of Penicillium purpurogenum in an aqueous / organic system

ZOU Shuping; ZHANG Qi; LI Limin; GUO Yanxia; DAI Dazhang; LI Chun

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Biosynthesis of glycyrrhetinic acid monoglucuronide by whole-cell of Penicillium purpurogenum in an aqueous / organic system

更新时间：2025-12-10

- Biosynthesis of glycyrrhetinic acid monoglucuronide by whole-cell of Penicillium purpurogenum in an aqueous / organic system
- CIESC Journal Issue 8, Pages: 2040-2045(2009)
- 作者机构：
  
  1. 北京理工大学生命学院,北京,100081
  2. 天津大学化工学院,天津,300072
- 作者简介：
- 基金信息：
- DOI：
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- Online First：05 August 2009，
  
  Published：05 August 2009
- 稿件说明：
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ZOU Shuping, ZHANG Qi, LI Limin, et al. Biosynthesis of glycyrrhetinic acid monoglucuronide by whole-cell of Penicillium purpurogenum in an aqueous / organic system[J]. CIESC Journal, 2009, (8): 2040-2045.
DOI：

ZOU Shuping, ZHANG Qi, LI Limin, et al. Biosynthesis of glycyrrhetinic acid monoglucuronide by whole-cell of Penicillium purpurogenum in an aqueous / organic system[J]. CIESC Journal, 2009, (8): 2040-2045. DOI：

摘要

研究了产紫青霉Penicillium purpurogenum Li-3 全细胞β-D-葡萄糖醛酸苷酶在水/有机溶剂体系中催化水解甘草酸，合成单葡糖醛酸基甘草次酸（GAMG）的反应。确定了最适反应体系为水/叔丁醇（体积比为3∶7），最适反应条件为：底物甘草酸用量1.5 g8226;L-1、反应温度45℃、pH值5.0、全细胞酶量7 g8226;L-1、摇床转速120 r8226;min-1，在此条件下反应48 h，产物GAMG产率可达79.12%，全细胞酶具有较好的稳定性和可重复使用性，显示了良好的应用前景。

Abstract

Biosynthesis of glycyrrhetinic acid monoglucuronide (GAMG) by β-D-glucuronidase from the whole cell of Penicillium purpurogenum Li-3 in an aqueous / organic system was studied.Results showed that tert-butyl alcohol is the best organic solvent and the optimum volume ratio of aqueous to organic phase is 3 to 7.The rest optimum conditions for this biocatalysis are 1.5 g8226;L-1 of substrate concentration

45℃ of temperature

pH 5.0

7 g8226;L-1 of enzyme concentration and 120 r8226;min-1 of shaking rate.Under these optimized conditions

79.12% yield of GAMG was achieved after 48 h reaction. The whole-cell enzyme has a good reusability and a high stability

which demonstrates the prospect for real application.

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