IMMOBILIZED CULTURE OF Bacillus subtilis IN SA/CS-CaCl<sub>2</sub>/PMCG MICROCAPSULE FOR PRODUCTION OF NATTOKINASE

MEI Lehe; ZHANG Xiaozhong; AI Biying; SHENG Qing; LIN Dongqiang; YAO Shanjing; ZHU Ziqiang

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IMMOBILIZED CULTURE OF Bacillus subtilis IN SA/CS-CaCl₂/PMCG MICROCAPSULE FOR PRODUCTION OF NATTOKINASE

更新时间：2025-12-10

- IMMOBILIZED CULTURE OF Bacillus subtilis IN SA/CS-CaCl₂/PMCG MICROCAPSULE FOR PRODUCTION OF NATTOKINASE
- CIESC Journal Issue 8, Pages: 1319-1323(2004)
- 作者机构：
  
  1. 浙江大学化学工程与生物工程系,浙江,杭州,310027
  2. 浙江省丽水市环境保护检测中心站,浙江,丽水,323000
  3. 浙江理工大学生命科学学院,浙江,杭州,310018
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：25 August 2004，
  
  Published：25 August 2004
- 稿件说明：
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MEI Lehe, ZHANG Xiaozhong, AI Biying, et al. IMMOBILIZED CULTURE OF Bacillus subtilis IN SA/CS-CaCl₂/PMCG MICROCAPSULE FOR PRODUCTION OF NATTOKINASE[J]. CIESC Journal, 2004, (8): 1319-1323.
DOI：

MEI Lehe, ZHANG Xiaozhong, AI Biying, et al. IMMOBILIZED CULTURE OF Bacillus subtilis IN SA/CS-CaCl₂/PMCG MICROCAPSULE FOR PRODUCTION OF NATTOKINASE[J]. CIESC Journal, 2004, (8): 1319-1323. DOI：

摘要

制备了海藻酸钠/纤维素硫酸钠-CaCl2/聚亚基二胍氯化氢微胶囊（SA/NaCS-CaCl2/PMCG微胶囊），并以该微胶囊固定化培养枯草杆菌HL-1用于发酵制备纳豆激酶，考察了PMCG对枯草杆菌生长的影响，比较了微囊化培养与游离培养过程中的菌体生长、耗糖速率以及纳豆激酶产物分泌能力.最后经过连续6批培养，酶活可达到2465 IU8226;ml-1，是游离培养过程的3倍.

Abstract

A SA/NaCS-CaCl2/PMCG microcapsule which can be used in biotechnology was prepared.The production of nattokinase from Bacillus subtilis immobilized in SA/CS-CaCl2/PMCG microcapsule was studied.The effects of PMCG on the growth of Bacillus subtilis were investgated.The growth rate

consumation rate of xylose and production rate of nottokinase of microencapsulated cells were compared with suspension culture.The results showed that immobilized cells grew as suspension culture for a single batch.The encapsulated fermentation had advantage in multi-batch culture.The highest activities of 2465 IU8226;ml-1 of nattokinase were reached

which was 2 times higher than that in suspension culture.

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IMMOBILIZED CULTURE OF Bacillus subtilis IN SA/CS-CaCl2/PMCG MICROCAPSULE FOR PRODUCTION OF NATTOKINASE

DOI：

摘要

Abstract

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IMMOBILIZED CULTURE OF Bacillus subtilis IN SA/CS-CaCl₂/PMCG MICROCAPSULE FOR PRODUCTION OF NATTOKINASE