IN-SITU SEPARATION BY MACROSPORE-ANIONIC EXCHANGE RESIN DURING RECOMBINANT E. coli CULTIVATION FOR hEGF PRODUCTION

CHEN Xin’ai; XU Zhinan; FAN Limei; CEN Peilin

您当前的位置：

首页 >

文章列表页 >

IN-SITU SEPARATION BY MACROSPORE-ANIONIC EXCHANGE RESIN DURING RECOMBINANT E. coli CULTIVATION FOR hEGF PRODUCTION

更新时间：2025-12-10

- IN-SITU SEPARATION BY MACROSPORE-ANIONIC EXCHANGE RESIN DURING RECOMBINANT E. coli CULTIVATION FOR hEGF PRODUCTION
- CIESC Journal Issue 3, Pages: 493-496(2004)
- 作者机构：
  
  1. 浙江大学生命科学学院,浙江,杭州,310029
  2. 浙江大学生物工程研究所,浙江,杭州,310027
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：25 March 2004，
  
  Published：25 March 2004
- 稿件说明：
移动端阅览
CHEN Xin’ai, XU Zhinan, FAN Limei, et al. IN-SITU SEPARATION BY MACROSPORE-ANIONIC EXCHANGE RESIN DURING RECOMBINANT E. coli CULTIVATION FOR hEGF PRODUCTION[J]. CIESC Journal, 2004, (3): 493-496.
DOI：

CHEN Xin’ai, XU Zhinan, FAN Limei, et al. IN-SITU SEPARATION BY MACROSPORE-ANIONIC EXCHANGE RESIN DURING RECOMBINANT E. coli CULTIVATION FOR hEGF PRODUCTION[J]. CIESC Journal, 2004, (3): 493-496. DOI：

摘要

Abstract

Production and accumulation of toxic by-products such as acetic acid can inhibit the growth of recombinant cells and the expression of exogenous gene in E.coli.An anionic exchange resin

A-D3-1

which is high in adsorption selectivity and capability for acetic acid

was screened from a variety of resins based on its physical and chemical properties.On the scale of shake flask culture

the addition of 1.0g resin per 30ml medium was insignificant for the cell growth

however

it could improve the hEGF expression significantly. The batch culture in 2.5L fermentor showed that in-situ adsorption of acetic acid by anionic exchange resin could enhance the expression level of interested protein and reduce the fermentation period by 2 hours. And up to 10％ improvement of hEGF (human epidermal growth factor)volumetric productivity (225.0mg8226;L-1) could be achieved by supplementing 3.3g resin per 100ml medium.

关键词

Keywords

references

Views

668

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Screening of cation exchange resins for treating wastewater containing high concentration ammonia

Preparation of high-pure choline

Study on flash evaporation characteristics as well as thermodynamic variation performance in liquid hydrogen tank during on-orbit venting

Analysis of flow field characteristics and mixing performance of different SK-type static mixers

Multi objective optimization of flow and heat transfer characteristics of modified manifold microchannel heat sink based on machine learning

Related Author

LI Hongyan

LI Yaxin

SUN Donggang

WANG Wei

ZHANG Xinsheng

Related Institution

太原理工大学环境科学与工程学院

化学工程联合国家重点实验室（华东理工大学）

⁰