ENZYMATIC SYNTHESIS OF 2-ETHYLHEXYL PALMITATE BY IMMOBILIZED candida sp.99-125 LIPASE

CHEN Biqiang; YE Hua; TAN Tianwei

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ENZYMATIC SYNTHESIS OF 2-ETHYLHEXYL PALMITATE BY IMMOBILIZED candida sp.99-125 LIPASE

更新时间：2025-12-10

- ENZYMATIC SYNTHESIS OF 2-ETHYLHEXYL PALMITATE BY IMMOBILIZED candida sp.99-125 LIPASE
- CIESC Journal Issue 3, Pages: 422-425(2004)
- 作者机构：
  
  北京化工大学化学工程学院,北京,100029
- 作者简介：
- 基金信息：
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  CLC：
- Online First：25 March 2004，
  
  Published：25 March 2004
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CHEN Biqiang, YE Hua, TAN Tianwei. ENZYMATIC SYNTHESIS OF 2-ETHYLHEXYL PALMITATE BY IMMOBILIZED candida sp.99-125 LIPASE[J]. CIESC Journal, 2004, (3): 422-425.
DOI：

CHEN Biqiang, YE Hua, TAN Tianwei. ENZYMATIC SYNTHESIS OF 2-ETHYLHEXYL PALMITATE BY IMMOBILIZED candida sp.99-125 LIPASE[J]. CIESC Journal, 2004, (3): 422-425. DOI：

摘要

开发了固定化假丝酵母脂肪酶99-125合成棕榈酸异辛酯的工艺.对反应温度、酶用量、底物摩尔比等酯化反应条件进行了研究.脂肪酶以吸附的形式固定在织物膜上，以1g棕榈酸、0.67g异辛醇、0.12g固定化脂肪酶和5ml石油醚组成的反应系统在40℃条件下反应24h，酯化率可达96.6%.固定化酶连续反应9批后酯化率仍维持在90%以上.

Abstract

The synthesis of 2-ethylhexyl palmitate by immobilized lipase candida sp. 99-125 was investigated.The lipase was immobilized on fiber membrane by adsorption. The conditions of enzymatic synthesis of 2-ethylhexyl palmitate were optimized. In the reaction system composed of 1g palmitic acid

0.67g 2-ethyl hexanol

5ml petroleum ether and 0.12g (1000IU) immobilized lipase

shaken in a 50ml conical flask at 40℃ for 24h

the esterification rate reached 97%. The reuse stability of immobilized lipase was about 9 batch.

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