L-ASPARAGINASE RELEASE FROM Escherichia coli CELLS WITH AQUEOUS TWO-PHASE SYSTEM

YU Jiaye; ZHAO Fengsheng

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L-ASPARAGINASE RELEASE FROM Escherichia coli CELLS WITH AQUEOUS TWO-PHASE SYSTEM

更新时间：2025-12-10

- L-ASPARAGINASE RELEASE FROM Escherichia coli CELLS WITH AQUEOUS TWO-PHASE SYSTEM
- CIESC Journal Issue 2, Pages: 264-268(2003)
- 作者机构：
  
  上海交通大学药学院
- 作者简介：
- 基金信息：
- DOI：
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- Online First：25 February 2003，
  
  Published：25 February 2003
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YU Jiaye, ZHAO Fengsheng. L-ASPARAGINASE RELEASE FROM Escherichia coli CELLS WITH AQUEOUS TWO-PHASE SYSTEM[J]. CIESC Journal, 2003, (2): 264-268.
DOI：

YU Jiaye, ZHAO Fengsheng. L-ASPARAGINASE RELEASE FROM Escherichia coli CELLS WITH AQUEOUS TWO-PHASE SYSTEM[J]. CIESC Journal, 2003, (2): 264-268. DOI：

摘要

Abstract

A method to release L-asparaginase(EC3.5.1.1) from Escherichia coli ATCC 11303 cells using an aqueous two-phase system was proposed. The system was composed of K2HPO4 and polyoxyethylene octyl phenyl ether (POOPE). This method combined enzyme release with enzyme purification.The influences of POOPE concentration

K2HPO4 concentration

E. coli cell concentration

pH and treatment time on the release of L-asparaginase were investigated.Under suitable conditions

over 90% of L-asparaginase in cells was released into surrounding medium.Electron micrography indicated that the chemical treatment altered the inner structure of E.coli cells significantly.

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