In order to eliminate the bacteria in inoculating sludge

which consume hydrogen

and to speed start-up as well as to rise efficiency of hydrogen production

effect of pretreatment temperatures of 65

80

95

110 and 121℃ for 30 min on hydrogen production from glucose was investigated in a batch cultures

with anaerobic granular sludge come from workshop treating wastewater of beer factory.The results come from fermentation showed that the amount of hydrogen produced

at conditions of initial pH=7.0

glucose concentration 10000 mg·L-1 and amount of inoculating sludge 2 g MLVSS·L-1

was less for untreated sludge than pretreated one.For anaerobic granular sludge treated at 95℃

the maximum accumulative hydrogen production in 120 h

133.47 ml

was obtained

and equal to hydrogen yields of 1.40 mol·mol-1 glucose

and specific hydrogen production of 15.93 mmol H2·(g MLVSS)-1. Although hydrogen production was varied for different pretreatment temperature

butyrate and acetate were dominated in soluble liquid products for all glucose fermentation tests

and their account was about 80% of the total volatile fatty acids and ethanol.