Effect of phospholipids and water contents on production of bio-diesel fuel catalyzed by lipases in a solvent system

LI Zebo; DU Wei; LIU Dehua

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Effect of phospholipids and water contents on production of bio-diesel fuel catalyzed by lipases in a solvent system

更新时间：2025-12-10

- Effect of phospholipids and water contents on production of bio-diesel fuel catalyzed by lipases in a solvent system
- CIESC Journal Issue 1, (2007)
- 作者机构：
  
  清华大学化学工程系
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：05 January 2007，
  
  Published：05 January 2007
- 稿件说明：
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LI Zebo, DU Wei, LIU Dehua. Effect of phospholipids and water contents on production of bio-diesel fuel catalyzed by lipases in a solvent system[J]. CIESC Journal, 2007, (1).
DOI：

LI Zebo, DU Wei, LIU Dehua. Effect of phospholipids and water contents on production of bio-diesel fuel catalyzed by lipases in a solvent system[J]. CIESC Journal, 2007, (1). DOI：

摘要

用叔丁醇作为反应介质，利用固定化脂肪酶催化油脂原料甲醇解反应制备生物柴油，消除了甲醇和甘油对酶的负面影响，酶的使用寿命显著延长，进一步系统研究了以叔丁醇作为反应介质，磷脂及水含量对脂肪酶催化特性的影响。当体系中中含水量达2%以上或磷脂含量达0.1%油重时将对固定化脂肪酶LipozymeIM TL 和Novozym 435的催化活性造成明显的不利影响。同时还发现把脂肪酶LipozymeIM TL 和 1%Novozym 435混合起来能大大提高脂肪酶的耐水、耐磷脂能力。

Abstract

tert-Butanol，as a novel reaction medium，was adopted for lipase-catalyzed trans-esterification of rapeseed oil for bio-diesel production，and the negative effects caused by excessive methanol and by-product glycerol could be eliminated.When the water content reached 2% or phospholipids content was more than 0.1% based on the oil mass，there would be obvious negative effects on lipase LipozymeIM TL and Novozym 435.Combined use of LipozymeIM TL and Novozym 435 was proposed further to catalyze the methanolysis，and the water-resistant and phospholipids-resistant abilities of the lipases could be improved.

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