Optimization of fermentation conditions for α-chloropropionic acid dehalogenases production based on response surface analysis

WANG Nengqiang; WU Jianping; ZOU Xiaoming; WANG Pu; YANG Lirong

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Optimization of fermentation conditions for α-chloropropionic acid dehalogenases production based on response surface analysis

更新时间：2025-12-10

- Optimization of fermentation conditions for α-chloropropionic acid dehalogenases production based on response surface analysis
- CIESC Journal Issue 1, (2007)
- 作者机构：
  
  1. 湖南科技大学生命科学学院
  2. 浙江大学材化学院
  3. 井冈山大学生命科学学院
  4. 浙江工业大学药学院
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：05 January 2007，
  
  Published：05 January 2007
- 稿件说明：
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WANG Nengqiang, WU Jianping, ZOU Xiaoming, et al. Optimization of fermentation conditions for α-chloropropionic acid dehalogenases production based on response surface analysis[J]. CIESC Journal, 2007, (1).
DOI：

WANG Nengqiang, WU Jianping, ZOU Xiaoming, et al. Optimization of fermentation conditions for α-chloropropionic acid dehalogenases production based on response surface analysis[J]. CIESC Journal, 2007, (1). DOI：

摘要

通过Plackett-Burman设计和响应面分析方法对Pseudomonas W20菌株产脱卤酶的培养基组成进行优化。得出影响产酶的3个重要的培养基成分为：尿素、葡萄糖和KH2PO4，且其最适浓度分别为1.19、18.4 g·L-1和1.30 g·L-1。此时脱卤酶活力达到了10.57 U·（g干菌体）-1，比优化前提高了23.77%。

Abstract

Two level factorials designs of Plackett-Burman were constructed to select the Pseudomonas W20 fermentation medium components of α-chloropropionic acid dehalogenases.Three important components were urea

glucose and KH2PO4.The optimized levels of these factors were determined by the response surface analysis. The results showed that the optimum concentrations of urea

glucose and KH2PO4 were 1.19 g·L-1

18.4 g·L-1 and 1.30 g·L-1 respectively.The enzyme activity increased by 23.77% under the optimized condition.

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