产1,3-丙二醇新型重组大肠杆菌JM109（pHsh-dhaB-yqhD）的构建及其转化甘油

<P>ZHANG Xiaomei; ZHUGE Bin; XIE Tao; TANG Xueming; FANG Huiying; ZHUGE Jian</P>

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产1,3-丙二醇新型重组大肠杆菌JM109（pHsh-dhaB-yqhD）的构建及其转化甘油

更新时间：2025-12-10

- 产1,3-丙二醇新型重组大肠杆菌JM109（pHsh-dhaB-yqhD）的构建及其转化甘油
- CIESC Journal Issue 12, (2006)
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- Online First：25 December 2006，
  
  Published：25 December 2006
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ZHANG Xiaomei, ZHUGE Bin, XIE Tao, et al. 产1,3-丙二醇新型重组大肠杆菌JM109（pHsh-dhaB-yqhD）的构建及其转化甘油[J]. CIESC Journal, 2006, (12).
DOI：

ZHANG Xiaomei, ZHUGE Bin, XIE Tao, et al. 产1,3-丙二醇新型重组大肠杆菌JM109（pHsh-dhaB-yqhD）的构建及其转化甘油[J]. CIESC Journal, 2006, (12).
DOI：

摘要

利用温控载体pHsh将编码甘油脱水酶的基因dhaB和编码1

3-丙二醇氧化还原酶同工酶的基因yqhD串联构建重组质粒pHsh-dhaB-yqhD

将其转化大肠杆菌得到新型产1

3-丙二醇重组大肠杆菌JM109(pHsh-dhaB-yqhD)

并对影响该重组菌发酵的营养因子进行研究.实验结果表明：该重组菌转化甘油合成1

3-丙二醇的适宜培养基组成为甘油60 g·L-1、酵母膏5.0 g·L-1、维生素B12 0.05 g·L-1以及KH2PO4 7.5 g·L-1; 以此培养基在5 L发酵罐上进行放大

3-丙二醇产量、转化率和生产能力分别达到43.26 g·L-1、72.2 %和1.55 g·L-1·h-1.

Abstract

The gene dhaB encoding glycerol dehydrase from Citrobacter freundii gene dhaB and 1

3-propanediol (1

3-PD)oxidoreductase isoenzyme encoding gene (yqhD) were transferred into temperature control expression vector pHsh to construct a novel recombinant Escherichia coli JM109 (pHsh-dhaB-yqhD).Some nutrient ingredients affecting fermentation results of this recombinant strain were also studied.The results demonstrated that the optimized medium comprised(g·L-1) glycerol 60

yeast extract 5.0

KH2PO4 7.5 and vitamin B12 0.05.The 1

3-propanediol concentration

yield on glycerol and productivity in the 5 L fermenter could reach 43.26 g·L-1

72.2 % and 1.55 g·L-1·h-1 respectively.

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