Mechanism and model of chiral separation of tryptophan by ultrafiltration using BSA(Ⅱ)

QI Xuan; Lü Yangcheng; CHEN Yang; LUO Guangsheng

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Mechanism and model of chiral separation of tryptophan by ultrafiltration using BSA(Ⅱ)

更新时间：2025-12-10

- Mechanism and model of chiral separation of tryptophan by ultrafiltration using BSA(Ⅱ)
- CIESC Journal Issue 1, (2007)
- 作者机构：
  
  清华大学化学工程国家重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：05 January 2007，
  
  Published：05 January 2007
- 稿件说明：
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QI Xuan, Lü Yangcheng, CHEN Yang, et al. Mechanism and model of chiral separation of tryptophan by ultrafiltration using BSA(Ⅱ)[J]. CIESC Journal, 2007, (1).
DOI：

QI Xuan, Lü Yangcheng, CHEN Yang, et al. Mechanism and model of chiral separation of tryptophan by ultrafiltration using BSA(Ⅱ)[J]. CIESC Journal, 2007, (1). DOI：

摘要

为了准确地预测以牛血清白蛋白（BSA）为手性分离剂的色氨酸（Trp）超滤分离效果，在以单一对映体进行大量实验得到相互作用参数的基础上，对BSA与色氨酸对映体3种不同的结合模型进行了检验和对比。结果表明：在7< pH <12的范围内，BSA与色氨酸的结合机理模型为单位点竞争模型。此模型可以对渗透液中D-色氨酸的浓度和分离系数进行较准确的预测。通过对实验数据进行拟合，给出了计算相互作用参数的计算公式。

Abstract

In order to accurately predict the effect of chiral separation

this work obtained several complexation constants from a large number of singular tryptophan (Trp) enantiomer experiments and compared three models of complexation mechanism of tryptophan to bovine serum albumin (BSA).In the pH range from 7 to 12

the complexation mechanism was a competitive binding of D- and L-tryptophan on a single site.Complexation constants (KD and KL) varied significantly by changing pH in the solution and the variation of KD and KL was caused by the change of conformation of BSA and electric charge characteristics of Trp.The model could well predict the concentration of D-tryptophan in the permeated solution and separation factor.The calculation equation of complexation constants was given by regressing experimental data to a certain function.

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