Effect of betaine on unfolding thermodynamics and refolding kinetics of lysozyme

刘建华 董晓燕 武扬 孙彦

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Effect of betaine on unfolding thermodynamics and refolding kinetics of lysozyme

更新时间：2025-12-10

- Effect of betaine on unfolding thermodynamics and refolding kinetics of lysozyme
- CIESC Journal Issue 2, (2007)
- 作者机构：
  
  天津大学化工学院生物化工系天津大学化工学院生物化工系天津大学化工学院生物化工系天津大学化工学院生物化工系
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- Online First：05 February 2007，
  
  Published：05 February 2007
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刘建华董晓燕武扬孙彦. Effect of betaine on unfolding thermodynamics and refolding kinetics of lysozyme[J]. CIESC Journal, 2007, (2).
DOI：

刘建华董晓燕武扬孙彦. Effect of betaine on unfolding thermodynamics and refolding kinetics of lysozyme[J]. CIESC Journal, 2007, (2). DOI：

摘要

应用荧光分析技术考察了甜菜碱对溶菌酶在盐酸胍溶液中稳定性的影响；并提出将甜菜碱作为添加剂应用于彻底变性还原溶菌酶的复性；同时应用表观竞争反应动力学模型分析了不同浓度甜菜碱对溶菌酶复性动力学的影响特性。结果表明，甜菜碱对溶菌酶具有稳定作用，可以作为添加剂促进变性还原溶菌酶的复性。甜菜碱可以抑制复性过程溶菌酶分子间的聚集，同时提高溶菌酶的复性速率，从而提高溶菌酶的复性收率。

Abstract

Equilibrium denaturation of lysozyme by guanidinium chloride in the presence of betaine was investigated by tryptophan fluorescenceBetaine was used as a folding aid to enhance the renaturation of denatured-reduced lysozyme

the refolding kinetic behavior was studied at a low guanidinium chloride concentration by a competitive model of first-order folding reaction and third-order aggregationIt was found that betaine could shift in the transition midpoint in the guanidinium chloride induced equilibrium unfolding experiments

indicating that betaine could improve the thermodynamic stability of lysozymeIn the presence of betaine

the aggregation rate was decreased and the refolding rate was increased

the refolding yield could be increased.

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