EFFECTS OF DIFFERENT SULFUR SOURCES ON GROWTH AND DESULFURIZATION ACTIVITY OF Nocardia globerula R-9

LUO Mingfang; XING Jianmin; GOU Zhongxuan; LI Shan; LIU Guoliang; XIE Yuchun; LIU Huizhou; CHEN Jiayong（Chia Yung Chen）

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EFFECTS OF DIFFERENT SULFUR SOURCES ON GROWTH AND DESULFURIZATION ACTIVITY OF Nocardia globerula R-9

更新时间：2025-12-10

- EFFECTS OF DIFFERENT SULFUR SOURCES ON GROWTH AND DESULFURIZATION ACTIVITY OF Nocardia globerula R-9
- CIESC Journal Issue 10, Pages: 1413-1417(2003)
- 作者机构：
  
  生化工程国家重点实验室、中国科学院过程工程研究所分离科学与工程青年实验室,北京,100080
- 作者简介：
- 基金信息：
- DOI：
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- Online First：25 October 2003，
  
  Published：25 October 2003
- 稿件说明：
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LUO Mingfang, XING Jianmin, GOU Zhongxuan, et al. EFFECTS OF DIFFERENT SULFUR SOURCES ON GROWTH AND DESULFURIZATION ACTIVITY OF Nocardia globerula R-9[J]. CIESC Journal, 2003, (10): 1413-1417.
DOI：

LUO Mingfang, XING Jianmin, GOU Zhongxuan, et al. EFFECTS OF DIFFERENT SULFUR SOURCES ON GROWTH AND DESULFURIZATION ACTIVITY OF Nocardia globerula R-9[J]. CIESC Journal, 2003, (10): 1413-1417. DOI：

摘要

用氯化钡沉淀结合傅里叶变换红外光谱法，证明小球诺卡氏菌（Nocardia globerula）R-9从二苯并噻吩中脱除的含硫产物以硫酸盐的形式存在于水相.比较了R-9分别以二苯并噻吩、硫酸钠和二甲基亚砜作为惟一硫源时的生长和脱硫活性.结果表明，二苯并噻吩、硫酸钠和二甲基亚砜均可以作为R-9的生长硫源，且高浓度（25 mmol8226;L-1）硫酸钠和二甲基亚砜对细胞的生长不产生抑制作用.2 mmol8226;L-1左右的硫酸钠或二甲基亚砜作为硫源时培养的细胞脱硫活性最高.提高硫源和碳源浓度可以提高R-9在发酵罐中的比生长速率和细胞浓度(OD600).

Abstract

Sulfate was identified as the final sulfur-containing product of DBT desulfurization by Nocardia globerula R-9 by means of precipitation with barium chloride and Fourier transform-infrared spectrometry. The growth and desulfurization activity of cells grown with DBT

sodium sulfate or dimethyl sulfoxide as the sulfur sources were investigated. The results showed that the said sulfur-containing compounds could be used as the sulfur sources of this strain. Furthermore

high concentration of sodium sulfate or dimethyl sulfoxide did not inhibit the growth of the strain. For the specific desulfurization activity

the optimal concentration of sulfur source was about 2 mmol8226;L-1.

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