Glycerol metabolism and methanol metabolism in genetically engineered Pichia pastoris were analyzed respectively in chemostat cultivation based on balances of oxygen and carbon elements.With the cell specific growth rate increasing from 0.064h-1 to 0.257h-1

the specific consumption rate of glycerol for cell growth metabolism increased from 1.36mmol8226;g-18226;h-1 to 5.46mmol8226;g-18226;h-1

whereas the percentage of glycerol metabolic flux for cell maintenance decreased from 14.4% to 4.0% when glycerol was the sole carbon source. The percentage of assimilation flux of glycerol increased by 5.9% while the percentage of catabolism flux of glycerol decreased by 5.9%

although the specific rates of assimilation and the specific rates of catabolism became larger.The recombinant human serum albumin was expressed in genetically engineered Pichia pastoris when methanol was used as substrate in continuous culture with specific growth rate increasing from 0.019h-1 to 0.046h-1.The largest specific consumption rate of methanol for target protein production was 0.31mmol8226;g-18226;h-1

which was only 13.2% of all methanol metabolic flux at μ=0.027h-1.