Improvement of porcine interferon-α production and ATP regeneration efficiency by reducing induction temperature

LIU Guoqiang; JIN Hu; GAO Minjie; DAI Keke; WANG Huihui; LI Zhen; SHI Zhongping

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Improvement of porcine interferon-α production and ATP regeneration efficiency by reducing induction temperature

更新时间：2025-12-10

- Improvement of porcine interferon-α production and ATP regeneration efficiency by reducing induction temperature
- CIESC Journal Issue 2, Pages: 444-451(2011)
- 作者机构：
  
  1. 江南大学生物工程学院
  2. 上海市农业科学院畜牧兽医研究所
  3. 上海市农业遗传育种重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：05 February 2011，
  
  Published：05 February 2011
- 稿件说明：
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LIU Guoqiang, JIN Hu, GAO Minjie, et al. Improvement of porcine interferon-α production and ATP regeneration efficiency by reducing induction temperature[J]. CIESC Journal, 2011, (2): 444-451.
DOI：

LIU Guoqiang, JIN Hu, GAO Minjie, et al. Improvement of porcine interferon-α production and ATP regeneration efficiency by reducing induction temperature[J]. CIESC Journal, 2011, (2): 444-451. DOI：

摘要

Abstract

The effect of induction temperatures on porcine interferon-α(pIFN-α）production byPichia pastoris was investigated in a 10 L bioreactor with methanol concentration controlled at a specific level. The results indicated that a low temperature was obviously beneficial for pIFN-α expression

and the highest pIFN-α antiviral activity reached a level of 1.5×106 IU·ml-1 when induction was made at 20℃

that was 150-folds of the maximal value obtained with the traditional induction strategy at 30℃.On-line multi-variable measurement of the fermentative state variables and metabolic analysis showed that cell metabolic activity and specific methanol consumption rate (0.036 h-1）could be higher with induction at 20 Key words: 毕赤酵母；猪αFONT-FAMILY: Calibri

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