A series of high-resolution CFD simulations as well as experiments were implemented to investigate the mixing in single stage and double stage T-shaped micro-channel networks.With Reynolds number increasing from 5 to 200

three different flow regimes

namely stratified flow

vortex flow and engulfment flow were observed.In the single stage T-shaped structure mixing efficiency was intensified only when engulfment flow regime was reached.While in the double stage T-shaped network structure

with contact area increasing and flow direction changing

mixing performance apparently improved after vortex flow appeared in the highest stage.Compared with single stage

mixing in the double stage structure occurred earlier.At the same residence time

mixing efficiency was higher in the double stage T-mixer than in the single stage one.Bionic networks containing multi-stage T-shaped micro-channel greatly enhance micro-mixing

and this kind of network is a powerful micro-mixing configuration.