Effect of redox potential regulation on metabolic flux distribution of succinate production by Actinobacillus succinogenes

JIANG Min; HUANG Xiumei; LI Jian; CHEN Kequan; YANG Zhuona; ZHENG Xiaoyu; WEI Ping

您当前的位置：

首页 >

文章列表页 >

Effect of redox potential regulation on metabolic flux distribution of succinate production by Actinobacillus succinogenes

更新时间：2025-12-10

- Effect of redox potential regulation on metabolic flux distribution of succinate production by Actinobacillus succinogenes
- CIESC Journal Issue 10, Pages: 2555-2561(2009)
- 作者机构：
  
  南京工业大学生物与制药工程学院，材料化学工程国家重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：05 October 2009，
  
  Published：05 October 2009
- 稿件说明：
移动端阅览
JIANG Min, HUANG Xiumei, LI Jian, et al. Effect of redox potential regulation on metabolic flux distribution of succinate production by Actinobacillus succinogenes[J]. CIESC Journal, 2009, (10): 2555-2561.
DOI：

JIANG Min, HUANG Xiumei, LI Jian, et al. Effect of redox potential regulation on metabolic flux distribution of succinate production by Actinobacillus succinogenes[J]. CIESC Journal, 2009, (10): 2555-2561. DOI：

摘要

利用代谢通量分析方法分析了氧化还原电位调控对产琥珀酸放线杆菌NJ113厌氧发酵产丁二酸过程的影响。结果表明，氧化还原电位调控可以改变HMP与EMP途径的代谢通量，从而影响胞内NADH/NAD+。在菌体最适氧化还原电位（-350 mV）条件下， HMP途径与EMP途径的通量比由43.6∶56.2提高至63.2∶36.5，间接合成更多的NADH，解决了丁二酸合成过程中还原力不足的矛盾，整个发酵过程中胞内NADH/NAD+比值有明显提高，发酵初期NADH/NAD+比值由2.19提高至8.73。最终丁二酸代谢通量从114.5 mmol.(g DCW)-1.h-1增至129.3 mmol.(g DCW)-1.h-1，副产物乙酸、甲酸的代谢通量分别降低30.6%、30.2%;丁二酸收率由75.46%提高至89.34%，生产强度达到1.18 g.L-1.h-1。

Abstract

The effect of redox potential regulation on metabolite flux distribution of Actinobacillus succinogenes NJ113 was studied by using the metabolic flux analysis methodThe results showed that the flux of HMP and EMP were changed after ORP regulationUnder the optimal ORP(-350 mV) condition

the ratio of HMP to EMP was improved from 43.6∶56.2 to 63.2∶36.5 and thus the reducing power was better balanced.The value of NADH/NAD+ at the ORP of -350 mV was much higher than that without regulationIn the initial phase the NADH/NAD+ was increased from 2.19 to 8.73.Finally

the succinic acid flux increased from 114.5 mmol.(g DCW)-1 .h-1 to 129.3 mmol.(g DCW)-1.h-1 with the metabolic flux of acetic acid and formic acid decreased by 30.6% and 30.2%

respectively.Succinic acid yield was increased from 75.46% to 89.34% with the productivity of 1.18 g.L-1.h-1.

关键词

Keywords

references

Views

337

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Sterilization characteristics of electrolyzed-oxidizing water and its sterilizing effect for meat

Kinetic models for anaerobic fermentation of butanedioic acid

Study on impact of impurities in coal chemical waste salt on solvation of NaCl and Na2SO4

Exothermic characteristics of energy carrier for calcium looping thermo-chemical energy storage

Study on the residence time distribution characteristics of the TPMS-Diamond static mixer

Related Author

ZHU Yuchan

REN Zhandong

LIU Ye

ZHANG Zhiyong

CHEN Kequan

JIANG Min

WEI Ping

SU Li

Related Institution

武汉工业学院

南京工业大学制药与生命科学学院，材料化学工程国家重点实验室

⁰