Preparation and structure morphology of tethered bilayer lipid membrane

LIU Aizhen; ZHANG Xiuling; QIU Yu’e; Dong Yan

您当前的位置：

首页 >

文章列表页 >

Preparation and structure morphology of tethered bilayer lipid membrane

更新时间：2025-12-10

- Preparation and structure morphology of tethered bilayer lipid membrane
- CIESC Journal Issue 6, Pages: 1600-1604(2009)
- 作者机构：
  
  德州学院化学系
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：05 June 2009，
  
  Published：05 June 2009
- 稿件说明：
移动端阅览
LIU Aizhen, ZHANG Xiuling, QIU Yu’e, et al. Preparation and structure morphology of tethered bilayer lipid membrane[J]. CIESC Journal, 2009, (6): 1600-1604.
DOI：

LIU Aizhen, ZHANG Xiuling, QIU Yu’e, et al. Preparation and structure morphology of tethered bilayer lipid membrane[J]. CIESC Journal, 2009, (6): 1600-1604. DOI：

摘要

利用表面硅烷化方法对化学氧化的单晶硅表面进行修饰，成功地将抗生物素蛋白固定在表面上，并且用囊泡融合法成功得到了大面积、连续的磷脂双层膜。由于成功地在基底与磷脂膜间引入了5nm左右的水层，原子力显微镜观察表明这种锚定的磷脂双层膜(tethered bilayer lipid membrane

tBLM)表现出与磷脂支持膜不同的相形为。两种膜相形为的不同可以用磷脂分子与基底间相互作用的不同来解释。

Abstract

Avidin was immobilized on chemically oxidized silicon substrate by means of the surface self-assembling method，and consecutive tethered lipid bilayer membrane was formed after vesicle fusion on such modified substrate.The structures of this tethered membrane were investigated by atomic force microscopy（AFM）.Due to the introduction of a 5 nm water cushion layer between the lipid bilayer and the substrate，a ripple phase was observed in this tethered membrane.The structure of such tethered membrane was different from the simple supported bilayer.These experimental observations demonstrated that the thickness increase of water-layer could effectively reduce the interaction between lipid bilayer and substrate.

关键词

Keywords

references

Views

167

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Study on dissolution kinetics of erythromycin thiocyanate

Progress of solar photo-thermo-catalysis for ammonia synthesis and decomposition under mild conditions

Thermo-gas-elastic coupling performance analysis of high-speed and high-temperature foil face seals

Heat transfer mechanisms, bottleneck identification and optimization in heat exchanger networks under variable operating conditions

Study on performance enhancement and cold retention characteristics of solid packed bed cold energy storage

Related Author

No data

Related Institution

No data

⁰