Extraction of extracellular polymeric substances from activated sludge in membrane bioreactor

ZHANG Hongwei; LEI Ming; LI Ying; ZHANG Xuehua; WANG Jie

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Extraction of extracellular polymeric substances from activated sludge in membrane bioreactor

更新时间：2025-12-10

- Extraction of extracellular polymeric substances from activated sludge in membrane bioreactor
- CIESC Journal Issue 6, Pages: 1531-1534(2008)
- 作者机构：
  
  天津大学环境科学与工程学院
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：05 June 2008，
  
  Published：05 June 2008
- 稿件说明：
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ZHANG Hongwei, LEI Ming, LI Ying, et al. Extraction of extracellular polymeric substances from activated sludge in membrane bioreactor[J]. CIESC Journal, 2008, (6): 1531-1534.
DOI：

ZHANG Hongwei, LEI Ming, LI Ying, et al. Extraction of extracellular polymeric substances from activated sludge in membrane bioreactor[J]. CIESC Journal, 2008, (6): 1531-1534. DOI：

摘要

采用七种方法（热、酸、碱处理等）提取MBR（Membrane Bioreactor）中污泥EPS（extracellular polymeric substances），研究了这些方法对于污泥EPS的最佳提取条件。在综合考虑各种方法的精确性、操作简易度、提取效果、对污泥细胞的破坏程度后，确定EDTA二钠提取（浓度2%，3h）和热提取（80℃，45min）是最简便有效的两种提取方法。NaOH提取（1mol.L-1，2h）对污泥细胞具有很大的破坏作用，投加甲醛（浓度2%，2h）之后提取核酸含量减少了21.5%，降低了NaOH溶液提取对于细胞的破坏作用。

Abstract

The optimum conditions and effectiveness of extraction of extracellular polymeric substances (EPS) from activated sludge in a membrane bioreactor（MBR） were studied under such conditions

thermal

acid

alkaline treatment methods

and it was concluded that both thermal treatment (80℃，45 min) and EDTA bi-sodium treatment were effective.The sludge cell was damaged seriously by NaOH treatment (1 mol·L-1

2 h)

but when formaldehyde was added

the nucleic acid concentration was reduced by 21.5%

and the damage to sludge cell was then decreased.

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