Hydrolysis of soybean isoflavone by immobilized β-glucosidase in a two phase system

ZHANG Tao; HUANG Zhe; LIN Zhanglin

您当前的位置：

首页 >

文章列表页 >

Hydrolysis of soybean isoflavone by immobilized β-glucosidase in a two phase system

更新时间：2025-12-10

- Hydrolysis of soybean isoflavone by immobilized β-glucosidase in a two phase system
- CIESC Journal Issue 2, Pages: 387-392(2008)
- 作者机构：
  
  清华大学化学工程系
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：05 February 2008，
  
  Published：05 February 2008
- 稿件说明：
移动端阅览
ZHANG Tao, HUANG Zhe, LIN Zhanglin. Hydrolysis of soybean isoflavone by immobilized β-glucosidase in a two phase system[J]. CIESC Journal, 2008, (2): 387-392.
DOI：

ZHANG Tao, HUANG Zhe, LIN Zhanglin. Hydrolysis of soybean isoflavone by immobilized β-glucosidase in a two phase system[J]. CIESC Journal, 2008, (2): 387-392. DOI：

摘要

采用共价交联的方法将β-葡萄糖苷酶固定到球形壳聚糖上，并对固定化酶的性质进行表征，得出固定化酶的最佳反应条件：pH=5，温度40℃。据此用乙酸乙酯和pH=5缓冲溶液的双相体系，在40℃条件下水解大豆异黄酮。与游离酶相比，固定化酶在此双相体系中起到了稳定酶的作用;与单相体系相比，双相体系中产物的产率显著提高，反应速率也更快，且能有效去除粗品大豆异黄酮的异味。对于30%左右级别的大豆异黄酮，水解的两个主要水解产物（大豆苷元和染料木素）的产率都能达到70%。

Abstract

β-Glucosidase was immobilized on chitosan beads by cross-linking with glutaraldehyde

and was characterized.The optimum pH and temperature were 5 and 40℃

respectively.Soybean isoflavone was hydrolyzed in a two-phase system containing ethyl acetate and aqueous solution (pH=5).In this system，immobilized enzyme was more stable compared to free enzyme

and faster reaction rates and higher yields were obtained compared to the corresponding aqueous solution.The yields of the two main hydrolysis products (daidzein and genistein) of soybean isoflavone were both around 70%.

关键词

Keywords

references

Views

215

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Progress in research of selective oxidation of low carbon polyols over nano-Au catalysts with precise electronic effect regulation

Research on the characterization of the molecular weight and its distribution of perfluoropolyether

A hybrid modeling and optimization strategy for sequential simulated moving bed

Optimization of heat storage and release cycle performance of Ca(OH)2/CaO indirect reactor

Prediction model for COD in petrochemical wastewater influent based on temporal-spatial dual-stream convolutional neural network

Related Author

No data

Related Institution

No data

⁰