Macroporous poly(glycidyl methacrylate-co-divinylbenzene)carrier containing epoxide group was prepared by bulk polymerization using a multiple porogen consisting of nano-granules of calcium carbonate as solid porogen and the mixture of toluene and heptane as liquid porogen.The results revealed that the carrier

with the porosity of 59.82% and specific surface area of 29.60 m·g-1

mainly contained two kinds of pores

macropores (150—400 nm

approximately 60%) and superpores (2000—8000 nm;about 14.9%).The epoxide groups were hydrolyzed in H+ atmosphere to afford hydroxyl group

which subsequently reacted with glutaraldehyde for the immobilization of lipase.The activities of lipase on different carriers were investigated by catalyzing hydrolysis of olive oil.The result showed that the immobilized lipase on the carrier with double pore structure showed the highest activity of catalyzing esterification of lauric acid.Furthermore

the esterification activity of immobilized lipase was also investigated by using the reaction of n-octanol and dodecanic acid in n-hexane.