Flexible immobilization of papain on amino polystyrene support

SHEN Bin; WEI Rongqing; LIU Xiaoning; ZHU Jianxing; OUYANG Pingkai

您当前的位置：

首页 >

文章列表页 >

Flexible immobilization of papain on amino polystyrene support

更新时间：2025-12-10

- Flexible immobilization of papain on amino polystyrene support
- CIESC Journal Issue 6, Pages: 1070-1075(2005)
- 作者机构：
  
  南京工业大学制药与生命科学学院,江苏,南京,210009
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：25 June 2005，
  
  Published：25 June 2005
- 稿件说明：
移动端阅览
SHEN Bin, WEI Rongqing, LIU Xiaoning, et al. Flexible immobilization of papain on amino polystyrene support[J]. CIESC Journal, 2005, (6): 1070-1075.
DOI：

SHEN Bin, WEI Rongqing, LIU Xiaoning, et al. Flexible immobilization of papain on amino polystyrene support[J]. CIESC Journal, 2005, (6): 1070-1075. DOI：

摘要

提出酶的“柔性固定化”模型，并以Mannich反应得到担载量为0.4～6.0 mmol NH28226;g-1的胺化聚苯乙烯树脂为载体，以双醛淀粉为柔性链，对木瓜蛋白酶进行柔性固定化，酶活回收率可达40%～50％，相当于手臂固定化酶活力回收率的1.8～2.4倍，且柔性固定化酶稳定性较好.该结果说明，酶的“柔性固定化”模型可以改善传统共价结合固定化酶及手臂固定化酶活力回收率不高的缺陷.

Abstract

In this paper the flexible immobilization model of enzyme is presented.A new amino polystyrene (0.4—6.0 mmol NH28226;g-1) was prepared by Mannich reaction as immobilization support

DAS was used as the flexible chain

and then papain was immobilized. The recovery of immobilized enzyme activity could reach 40%—50%

equal to 1.8—2.4 times that of arm spacer support

and the stability of immobilized enzyme was good.The flexible immobilized model could achieve a higher recovery of immobilized enzyme activity than traditional covalent immobilization and arm spacer immobilization.

关键词

Keywords

references

Views

187

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Heat transfer mechanisms, bottleneck identification and optimization in heat exchanger networks under variable operating conditions

Study on entropy wave production and dissipation in partially premixed swirl flames subject to inlet perturbations

The impact of novel flow channel structure with sudden-expansion ratio on seawater-activated battery

Recent advances and perspectives on sulfide-based photocatalysts for hydrogen production

Optimization of heat storage and release cycle performance of Ca(OH)2/CaO indirect reactor

Related Author

No data

Related Institution

No data

⁰