Regulation of sodium citrate on metabolic flux distribution ofinosine fermentation process

CHEN Shuangxi; HUANG Mingzhi; CHU Ju; ZHUANG Yingping; ZHANG Siliang

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Regulation of sodium citrate on metabolic flux distribution ofinosine fermentation process

更新时间：2025-12-10

- Regulation of sodium citrate on metabolic flux distribution ofinosine fermentation process
- CIESC Journal Issue 9, Pages: 1731-1737(2005)
- 作者机构：
  
  1. 华东理工大学生物反应器工程国家重点实验室,上海,200237
  2. 河南大学生命科学学院,河南,开封,475001
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：25 September 2005，
  
  Published：25 September 2005
- 稿件说明：
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CHEN Shuangxi, HUANG Mingzhi, CHU Ju, et al. Regulation of sodium citrate on metabolic flux distribution ofinosine fermentation process[J]. CIESC Journal, 2005, (9): 1731-1737.
DOI：

CHEN Shuangxi, HUANG Mingzhi, CHU Ju, et al. Regulation of sodium citrate on metabolic flux distribution ofinosine fermentation process[J]. CIESC Journal, 2005, (9): 1731-1737. DOI：

摘要

基于物料的质量守恒定律和中间代谢物的拟稳态假设，定量研究了柠檬酸钠对肌苷发酵过程不同时段代谢流分布的影响.结果表明，添加的柠檬酸钠能够抑制存在于EMP途径和TCA循环之间的“碳源溢流”.在24 h以后， EMP途径的代谢通量平均降低33％，HMP途径通量平均增加76％，肌苷合成途径通量平均增加77％，最终产苷由17.44 g8226;L-1提高到24 g8226;L-1

增幅达37.6％.

Abstract

Metabolic flux analysis was used to understand macroscopical characteristics of fermentation process and cellular metabolic regulation.Based on the law of mass conservation and the assumption of pseudo-steady-state for the intermediates in the metabolic pathways

the effect of sodium citrate on the fluxes distribution of inosine fermentation process was quantitatively analyzed.It was revealed that carbon overflow between EMP pathways and TCA cycle could be restrained with the medium supplemented with sodium citrate. After 24 hours

the flux of EMP pathway decreased by 33% averagely

the flux of HMP pathway increased by 76% averagely

and the flux of inosine synthesis pathway increased by 77% on average.The final inosine yield was improved from 17.44 g8226;L-1 to 24 g8226;L-1

by an increase of 37.6%.

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