APPLICATION OF LIQUID CHROMATOGRAPHY FOR ISOLATION AND PURIFICATION OF SOLID-PHASE SYNTHETIC THYMOSIN α1

GAN Yiru; HUANG Yongdong; WU Lei

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APPLICATION OF LIQUID CHROMATOGRAPHY FOR ISOLATION AND PURIFICATION OF SOLID-PHASE SYNTHETIC THYMOSIN α1

更新时间：2025-12-10

- APPLICATION OF LIQUID CHROMATOGRAPHY FOR ISOLATION AND PURIFICATION OF SOLID-PHASE SYNTHETIC THYMOSIN α1
- CIESC Journal Issue 3, Pages: 497-500(2004)
- 作者机构：
  
  天津大学化工学院,天津,300072
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：25 March 2004，
  
  Published：25 March 2004
- 稿件说明：
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GAN Yiru, HUANG Yongdong, WU Lei. APPLICATION OF LIQUID CHROMATOGRAPHY FOR ISOLATION AND PURIFICATION OF SOLID-PHASE SYNTHETIC THYMOSIN α1[J]. CIESC Journal, 2004, (3): 497-500.
DOI：

GAN Yiru, HUANG Yongdong, WU Lei. APPLICATION OF LIQUID CHROMATOGRAPHY FOR ISOLATION AND PURIFICATION OF SOLID-PHASE SYNTHETIC THYMOSIN α1[J]. CIESC Journal, 2004, (3): 497-500. DOI：

摘要

Abstract

By using ion-exchange preparative chromatography (IEPC) and reversed-phase high performance liquid preparative chromatography(RP-HPLPC)

thymosin α1 was isolated and purified from the crude product synthesized by the solid-phase peptide synthesis(SPPS) method. The purity of the final product reached 95% through ion-exchange chromatography on DEAE Sepharose Fast Flow chromatography and Delta-PakTM C18 purification after optimizing the chromatographic conditions. The capacity of purification process was 50mg/circle. The total yield was 36%. The technology is simple and reliable

and can be scaled up easily.

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