RENATURATION OF RECOMBINANT HUMAN INTERFERON-γ INCLUSION BODY BY DILUTION

JIN Ting; GUAN Yixin; FEI Zhengzheng; YAO Shanjing

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RENATURATION OF RECOMBINANT HUMAN INTERFERON-γ INCLUSION BODY BY DILUTION

更新时间：2025-12-10

- RENATURATION OF RECOMBINANT HUMAN INTERFERON-γ INCLUSION BODY BY DILUTION
- CIESC Journal Issue 5, Pages: 770-774(2004)
- 作者机构：
  
  浙江大学化学工程与生物工程学系,浙江,杭州,310027
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：25 May 2004，
  
  Published：25 May 2004
- 稿件说明：
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JIN Ting, GUAN Yixin, FEI Zhengzheng, et al. RENATURATION OF RECOMBINANT HUMAN INTERFERON-γ INCLUSION BODY BY DILUTION[J]. CIESC Journal, 2004, (5): 770-774.
DOI：

JIN Ting, GUAN Yixin, FEI Zhengzheng, et al. RENATURATION OF RECOMBINANT HUMAN INTERFERON-γ INCLUSION BODY BY DILUTION[J]. CIESC Journal, 2004, (5): 770-774. DOI：

摘要

采用稀释法研究了重组人γ-干扰素包涵体复性条件，如温度、pH值、蛋白浓度、复性液中脲浓度，同时对加样操作方式进行了考察，得到了较为适宜的复性条件.结果表明，脲能有效地抑制复性过程中蛋白质的聚集，合适的脲浓度随复性蛋白浓度的增加而有所增大，在4℃、pH值8.0、脲浓度1.0mol8226;L-1、蛋白浓度0.1mg8226;ml-1及脉冲加样操作方式等条件下，复性后重组人γ-干扰素的活性为2.39×105 IU8226;ml-1，比活达2.21×107 IU8226;mg-1.

Abstract

The experimental conditions

including temperature

denatured protein concentration and urea concentration

in the renaturation process of recombinant human interferon-γ inclusion body by dilution were studied and at the same time pulse renaturation was tested. The optimum renaturation conditions were obtained. Results showed that urea could suppress the aggregation of protein during the renaturation process and the suitable urea concentration increased with the increase of denatured protein concentration. The activity and specific bioactivity was up to 2.39×105 IU8226;ml-1 and 2.21×107 IU8226;mg-1 respectively under 4 ℃

pH 8.0

1.0 mol8226;L-1 urea

0.1 mg8226;ml-1 protein and pulse renaturation operation mode.

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