MODELING OF HOLLOW-FIBER MEMBRANE PARTITIONING CHROMATOGRAPHY OF AMINO ACID

BAI Shu; XUE Bo; SHI Qinghong; SUN Yan

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MODELING OF HOLLOW-FIBER MEMBRANE PARTITIONING CHROMATOGRAPHY OF AMINO ACID

更新时间：2025-12-10

- MODELING OF HOLLOW-FIBER MEMBRANE PARTITIONING CHROMATOGRAPHY OF AMINO ACID
- CIESC Journal Issue 7, Pages: 978-983(2003)
- 作者机构：
  
  天津大学化工学院生物化工系,天津,300072
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Online First：25 July 2003，
  
  Published：25 July 2003
- 稿件说明：
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BAI Shu, XUE Bo, SHI Qinghong, et al. MODELING OF HOLLOW-FIBER MEMBRANE PARTITIONING CHROMATOGRAPHY OF AMINO ACID[J]. CIESC Journal, 2003, (7): 978-983.
DOI：

BAI Shu, XUE Bo, SHI Qinghong, et al. MODELING OF HOLLOW-FIBER MEMBRANE PARTITIONING CHROMATOGRAPHY OF AMINO ACID[J]. CIESC Journal, 2003, (7): 978-983. DOI：

摘要

以色氨酸为例，考虑氨基酸解离和在中空纤维膜内扩散传质建立了氨基酸中空纤维膜分配色谱理论模型.该模型采用完全独立的模型参数，模拟结果与实验数据吻合较好，验证了模型的可靠性.理论计算表明流动相的pH值与局部氨基酸浓度密切相关；理论和实验结果表明，流动相离子强度对氨基酸的保留行为有很大影响，采用梯度洗脱的方式可以改善洗脱峰形，提高色谱柱的分离度.

Abstract

A theoretical model for hollow-fiber membrane partition chromatography of amino acid was established by taking into account the dissociation of amino acid and mass transfer in the stationary phase immobilized in the membrane. With all the parameters determined independently

the model simulated very well the experimental chromatograms of a model amino acid

L-tryptophan

indicating the applicability of the model. Theoretical analysis also revealed the dependence of mobile phase pH on local amino acid concentration. In addition

the experimental and simulation results showed that mobile phase ionic strength had significant effect on the retention and peak shape of amino acid. Using a gradient elution mode can improve the peak shape and increase the column efficiency.

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